Mouse Anti-RB1 Recombinant Antibody (ZG-060R)
CAT#: ZG-060R
This product is a mouse antibody that recognizes RB1. It can be used in a wide variety of applications, such as: ELISA, IHC.
Tested Data
Gene Expression
IHC
Figure 1 Mouse Anti-RB1 Antibody (ZG-060R) in IHC
Immunohistochemistry staining of paraffin-embedded human normal esophagus (A) and stomach (B) tissue, showing nucleus localization withDAB staining using Rb monoclonal antibody.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/95/656_B4_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139687-RB1
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Specifications
- Immunogen
- Purified recombinant fragment of human Rb expressed in E. coli
- Host Species
- Mouse
- Type
- Mouse IgG
- Species Reactivity
- Human
- Applications
- ELISA, IHC
Product Property
- Purification
- Affinity purified
- Purity
- >95%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- IHC: 1:200-1:1000, ELISA: 1:10000
Target
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Related Diseases
EGFR Tyrosine Kinase Inhibitor Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "RB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1108MZ | Recombinant Mouse Anti-Human RB1 Antibody (clone 283D2005) | WB | Mouse antibody |
| ZG-0915F | Mouse Anti-RB1 Recombinant Antibody (clone XZ91) | WB, IP | Mouse IgG1 |
| ZG-0543U | Rabbit Anti-Phospho-RB1 (S807) Recombinant Antibody (clone 1H3) | ELISA, IF | Rabbit IgG |
| ZG-0544U | Rabbit Anti-Phospho-RB1 (S780) Recombinant Antibody (clone 2E9) | ELISA, IHC, IF, IP | Rabbit IgG |
| VS3-WK2044 | Rabbit Anti-RB1 (pT356) Recombinant Antibody (clone R04-1C4) | WB, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3015 | Hi-Affi™ Recombinant Rabbit Anti-RB1 Monoclonal Antibody (DS3015AB) | ICC, IF, IP, WB | IgG |
| MOR-4704 | Hi-Affi™ Recombinant Rabbit Anti-RB1 Monoclonal Antibody (TH218DS) | WB, IF, ICC, FC | IgG |
| MOR-4705 | Hi-Affi™ Recombinant Rabbit Anti-RB1 Monoclonal Antibody (TH219DS) | WB, IF, ICC, FC | IgG |
| MOR-4706 | Hi-Affi™ Recombinant Rabbit Anti-RB1 Monoclonal Antibody (TH220DS) | WB, IF, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6011 | Anti-RB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6012 | Anti-Mouse RB1 Immunohistochemistry Kit | IHC |
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