Rat Anti-RBPJ Recombinant Antibody (clone 0725W34)
CAT#: VS-0725-FY194
This recombinant antibody, clone 0725W34, targets full-length recombinant human RBPJ protein. It is validated for Chromatin Immunoprecipitation (ChIP), Electrophoretic Mobility Shift Assay (EMSA), Immunocytochemistry (ICC), and Immunofluorescence (IF). RBPJ is a transcriptional regulator important in the Notch signaling pathway, acting as a repressor or activator depending on Notch binding. This antibody is valuable for studying gene regulation, chromatin remodeling, and developmental processes.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60647/1227_G4_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows strong positivity in neuropil.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/251/1013_B_8_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/251/1013_B_8_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/251/1013_A_4_6.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000168214-RBPJ
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Specifications
- Immunogen
- Full-length recombinant protein corresponding to human RBPJ.
- Host Species
- Rat
- Type
- Rat IgG2b
- Specificity
- Human RBPJ
- Species Reactivity
- Human, Mouse
- Clone
- 0725W34
- Applications
- Chromatin Immunoprecipitation, Electrophoretic Mobility Shift Assay, Immunocytochemistry, Immunofluorescence
- Related Disease
- Cancer, Developmental disorders
Product Property
- Purification
- Protein G purified
- Molecular Weight
- The molecular weight of the antigen is 68 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunocytochemistry/Immunofluorescence: 0.5 µg/mL
Target
- Alternative Names
- Recombination Signal Binding Protein For Immunoglobulin Kappa J Region; Renal Carcinoma Antigen NY-REN-30; RBP-J Kappa; IGKJRB1; RBPSUH; IGKJRB; RBP-JK; CBF-1; RBP-J; RBPJK; Immunoglobulin Kappa J Region Recombination Signal Binding Protein 1; Recombining Binding Protein Suppressor Of Hairless (Drosophila)
- Gene ID
- 3516
- UniProt ID
- Q06330
- Long Name
- Recombination Signal Binding Protein For Immunoglobulin Kappa J Region
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "RBPJ"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3404z | Mouse Anti-RBPJ Recombinant Antibody (clone 1B11) | IHC, IF, WB, FC | Mouse IgG1 |
| MOB-1780CT | Recombinant Mouse anti-Human RBPJ Monoclonal antibody (EML477) | WB | |
| MRO-1326-CN | Recombinant Rabbit Anti-RBPJ Monoclonal Antibody (JB49-34) | WB | Rabbit IgG |
| VS3-FY1260 | Recombinant Rabbit Anti-RBPJ Antibody (clone R05-6G4) | WB | Rabbit IgG |
| VS13-YC993 | Rabbit Anti-RBPJ Recombinant Antibody (VS13-YC993) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3030 | Hi-Affi™ Recombinant Rabbit Anti-RBPJ Monoclonal Antibody (DS3030AB) | WB, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1896 | Anti-RBPJ Immunohistochemistry Kit | IHC | |
| VS-0525-XY6049 | Anti-Human RBPJ Immunohistochemistry Kit | IHC |
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