Mouse Anti-RDH12 Recombinant Antibody (clone 1E11)

CAT#: VS-0625-FY69

This Mouse IgG2a kappa monoclonal antibody, clone 1E11, is designed for detecting human RDH12. Its immunogen is a partial recombinant human RDH12 protein, with an epitope spanning amino acids 1-90. With an approximate antigen size of 63.1 kDa, this antibody is suitable for Western Blot, ELISA, Immunocytochemistry, and Immunofluorescence (10 µg/mL), providing versatile detection capabilities for RDH12.

Specifications

  • Immunogen
  • A partial recombinant human RDH12 protein (NP_689656.1, amino acids 217-316) with a GST tag (26 kDa).
  • Host Species
  • Mouse
  • Type
  • Mouse IgG2a, kappa
  • Specificity
  • Reacts with retinol dehydrogenase 12 (all-trans/9-cis/11-cis isoforms).
  • Species Reactivity
  • Human
  • Clone
  • 1E11
  • Applications
  • Western Blot, ELISA, Immunocytochemistry, Immunofluorescence
  • Related Disease
  • Leber Congenital Amaurosis (LCA), Retinitis Pigmentosa (RP).

Product Property

  • Purification
  • Purified with Protein A or G affinity chromatography
  • Purity
  • >90% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS, pH 7.4
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • Immunofluorescence: 10 µg/mL
    Optimal dilutions for assays should be determined by the user.

Target

  • Alternative Names
  • Retinol Dehydrogenase 12 (All-Trans/9-Cis/11-Cis); Short Chain Dehydrogenase/Reductase Family 7C Member 2; All-Trans And 9-Cis Retinol Dehydrogenase; SDR7C2; Short Chain Dehydrogenase/Reductase Family 7C, Member 2; Retinol Dehydrogenase 12 (All-Trans And 9-Cis); Retinol Dehydrogenase 12, All-Trans And 9-Cis
  • Long Name
  • Retinol Dehydrogenase 12 (All-Trans/9-Cis/11-Cis)
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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