Rabbit Anti-RPS6KB1 Recombinant Antibody (clone 1H8)
CAT#: ZG-0010U
The purified Rabbit Anti-RPS6KB1 Recombinant Antibody (clone 1H8) displayed high specificity against the RPS6KB1 protein of human and mouse.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-RPS6KB1 Antibody (ZG-0010U) in WB
Positive WB detected in: NIH/3T3 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate, Mouse Brain whole cell lysate All lanes: S6K1 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60, 57, 53, 57, 52 kDa
Observed band size: 70 kDa
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39442/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows distinct nuclear positivity in hematopoietic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39442/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3838/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human stomach
Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/ihc_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_9_8.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (MCF7)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108443-RPS6KB1
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Specifications
- Immunogen
- A synthesized peptide derived from human S6K1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human RPS6KB1
- Species Reactivity
- Human, Mouse
- Clone
- 1H8
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
- Related Disease
- Tuberous Sclerosis and Lymphangioleiomyomatosis
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot. Specific conditions should be optimized by the end user.
WB:1:500-1:5000
Target
- Alternative Names
- Ribosomal protein S6 kinase beta-1;S6K-beta-1; S6K1; EC 2.7.11.1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA; RPS6KB1; STK14A
- Gene ID
- 6198
- UniProt ID
- P23443
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "RPS6KB1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3126 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (DS3126AB) | IHC-P | IgG |
| MOR-4660 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (TH174DS) | WB, IF, ICC, FC | IgG |
| MOR-4699 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (TH213DS) | WB, IF, ICC, FC | IgG |
| MOR-4700 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (TH214DS) | WB, FC, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0167F | Mouse Anti-RPS6KB1 Recombinant Antibody (clone 1C7) | IHC-P, IF | Mouse IgG |
| VS3-QX985 | Mouse Anti-RPS6KB1 Recombinant Antibody (clone 3A2-2E7-2D4) | IHC | Mouse IgG1 |
| VS3-QX986 | Mouse Anti-RPS6KB1 Recombinant Antibody (clone 8H8) | IHC | Mouse IgG1 |
| VS3-XY3490 | Rabbit Anti-RPS6KB1 Recombinant Antibody (clone R07-7V3) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| VS3-WK2109 | Rabbit Anti-RPS6KB1 (pT421/pS424) Recombinant Antibody (clone R09-3B7) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1968 | Anti-RPS6KB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6231 | Anti-Mouse RPS6KB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6230 | Anti-Human RPS6KB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6232 | Anti-Rat RPS6KB1 Immunohistochemistry Kit | IHC |
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