Rabbit Anti-Phospho-RPS6KB1 (T421+S424) Recombinant Antibody (clone 3B6) (CAT#: ZG-0555U)
Rabbit Anti-Phospho-RPS6KB1 (T421+S424) Antibody (clone 3B6) displayed high specificity against the RPS6KB1 protein of Human.
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WB
Figure 1 Rabbit Anti-Phospho-RPS6KB1 (T421+S424) Antibody (ZG-0555U) in WB
Western Blot
Positive WB detected in 293 whole cell lysate(treated with Calyculin A or not)
All lanes Phospho-RPS6KB1 antibody at 0.93μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 70 KDa
Observed band size: 70 KDa
IF
Figure 2 Rabbit Anti-Phospho-RPS6KB1 (T421+S424) Antibody (ZG-0555U) in IF
Immunofluorescence staining of Hela cells with ZG-0555U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
IP
Figure 3 Rabbit Anti-Phospho-RPS6KB1 (T421+S424) Antibody (ZG-0555U) in IP
Immunoprecipitating Phospho-RPS6KB1 in Hela whole cell lysate
Lane 1: Rabbit control IgG(1μg)instead of ZG-0555U in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0555U(3μg)+ Hela whole cell lysate(1mg)
Lane 3: Hela whole cell lysate (20μg)
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-RPS6KB1 (T421+S424)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-RPS6KB1 (T421+S424)
- Species Reactivity
- Human
- Clone
- 3B6
- Applications
- ELISA, WB, IF, IP
- Conjugate
- Unconjugated
- Related Disease
- Tuberous Sclerosis and Lymphangioleiomyomatosis.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:20-1:200, IP:1:200-1:1000
Target
- Alternative Names
- Ribosomal protein S6 kinase beta-1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA; RPS6KB1; STK14A
- Gene ID
- 6198
- UniProt ID
- P23443
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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|---|---|---|---|
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| VS3-XY1387 | Mouse Anti-RPS6KB1 Recombinant Antibody (clone 5G9) | ELISA, WB, FC, IHC | Mouse IgG1 |
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| VS3-FY1285 | Recombinant Rabbit Anti-RPS6KB1 Antibody (clone R03-2B2) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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