Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (TH174DS)
CAT#: MOR-4660
Recombinant Rabbit anti-RPS6KB1 monoclonal antibody for WB, IF, ICC, FCM, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39442/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows distinct nuclear positivity in hematopoietic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39442/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3838/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human stomach
Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/ihc_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_9_8.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (MCF7)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108443-RPS6KB1
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Specifications
- Immunogen
- Peptide corresponding to amino acids 294-306 of human p70 Ribosomal Protein S6 Kinase
- Host Species
- Rabbit
- Type
- IgG
- Species Reactivity
- Mouse, Human
- Clone
- TH174DS
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunocytochemistry; Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Applications
- Application Notes
- FC: 1:100-1:500; ICC: 1:100-1:500; IF: 1:100-1:500; WB: 1:500-1:5000
Target
- Alternative Names
- Ribosomal Protein S6 Kinase B1; Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1; Serine/Threonine-Protein Kinase 14A; Ribosomal Protein S6 Kinase I; EC 2.7.11.1; S6K-Beta-1; P70 S6KA; STK14A; S6K1; Ribosomal Protein S6 Kinase, 70kD, Polypeptide 1; 70 KDa Ribosomal Protein S6 Kinase 1; Serine/Threonine Kinase 14 Alpha; P70 Ribosomal S6 Kinase Alpha; P70 S6 Kinase, Alpha; P70 S6 Kinase Alpha; P70(S6K)-Alpha; P70 S6K-Alpha; P70-Alpha; P70-S6K 1; EC 2.7.11; P70-S6K; P70S6K1; PS6K; S6K
- Gene ID
- 6198
- UniProt ID
- P23443
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "RPS6KB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3126 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (DS3126AB) | IHC-P | IgG |
| MOR-4699 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (TH213DS) | WB, IF, ICC, FC | IgG |
| MOR-4700 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (TH214DS) | WB, FC, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0010U | Rabbit Anti-RPS6KB1 Recombinant Antibody (clone 1H8) | ELISA, WB | Rabbit IgG |
| ZG-0054U | Rabbit Anti-RPS6KB1 Recombinant Antibody (clone 7D4) | ELISA, IF | Rabbit IgG |
| ZG-0555U | Rabbit Anti-Phospho-RPS6KB1 (T421+S424) Recombinant Antibody (clone 3B6) | ELISA, WB, IF, IP | Rabbit IgG |
| VS3-QX985 | Mouse Anti-RPS6KB1 Recombinant Antibody (clone 3A2-2E7-2D4) | IHC | Mouse IgG1 |
| VS3-QX986 | Mouse Anti-RPS6KB1 Recombinant Antibody (clone 8H8) | IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1968 | Anti-RPS6KB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6231 | Anti-Mouse RPS6KB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6230 | Anti-Human RPS6KB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6232 | Anti-Rat RPS6KB1 Immunohistochemistry Kit | IHC |
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