This product is antibody recognizes RSV. The antibody 6H5/H1 immunoassay techniques such as: ELISA.
Figure 1 Determination of sensitivity of monoclonal antibodies produced by the hybridomas 2E6/D2 and 6H5/H1 in the detection of the protein P of RSV.
ELISA plates were activated with serial dilutions 1:2, starting with 50 ng of protein P and finalizing with 0.04 ng (A) and serial dilutions 1:2 starting with an inoculum 1x10⁵ pfu of RSV to the dilution 1:5. 120 (B). Unactivated wells were included as negative control. The data shown in the plot chart express the absorbance to 450 nm emitted by the Horseradish peroxidase (HRP) enzyme present in the anti-P antibodies from the hybridomas 2E6/D2 and 6H5/H1, which were used in amount of 170 ng/well (A and B). The anti-P RSVH102 of RSV, catalogue number #AB94965, of Abcam, was also used in a concentration of 170 ng/well (A and B). The values correspond to the average of the emitted absorbance for each sample, in at least two independent assays.
Figure 2 Serial dilutions assay of the RSV anti-P monoclonal antibodies produced by the hybridomas 2E6/D2 and 6H5/H1, for the RSV purified antigens detection.
ELISA plates were activated with 50 ng of RSV recombinant protein P and the antigen was detected with serial dilutions 1:2 of the anti-P antibodies 2E6/D1 or 6H5/H1, starting from a concentration of 3.4 g/ml (170 ng/well). The data is expressed as the average of the emitted absorbance value to 450 nm of each sample in duplicate, in at least two independents assays.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
For research use only. Not intended for any clinical use.