Recombinant Mouse Anti-RSV Antibody (6H5/H1) (CAT#: HPAB-2538LY)

This product is antibody recognizes RSV. The antibody 6H5/H1 immunoassay techniques such as: ELISA.

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ELISA

Figure 1 Determination of sensitivity of monoclonal antibodies produced by the hybridomas 2E6/D2 and 6H5/H1 in the detection of the protein P of RSV.

ELISA plates were activated with serial dilutions 1:2, starting with 50 ng of protein P and finalizing with 0.04 ng (A) and serial dilutions 1:2 starting with an inoculum 1x10⁵ pfu of RSV to the dilution 1:5. 120 (B). Unactivated wells were included as negative control. The data shown in the plot chart express the absorbance to 450 nm emitted by the Horseradish peroxidase (HRP) enzyme present in the anti-P antibodies from the hybridomas 2E6/D2 and 6H5/H1, which were used in amount of 170 ng/well (A and B). The anti-P RSVH102 of RSV, catalogue number #AB94965, of Abcam, was also used in a concentration of 170 ng/well (A and B). The values correspond to the average of the emitted absorbance for each sample, in at least two independent assays.

ELISA

Figure 2 Serial dilutions assay of the RSV anti-P monoclonal antibodies produced by the hybridomas 2E6/D2 and 6H5/H1, for the RSV purified antigens detection.

ELISA plates were activated with 50 ng of RSV recombinant protein P and the antigen was detected with serial dilutions 1:2 of the anti-P antibodies 2E6/D1 or 6H5/H1, starting from a concentration of 3.4 g/ml (170 ng/well). The data is expressed as the average of the emitted absorbance value to 450 nm of each sample in duplicate, in at least two independents assays.


Specifications

  • Host Species
  • Mouse
  • Specificity
  • RSV
  • Species Reactivity
  • RSV
  • Clone
  • 6H5/H1
  • Applications
  • ELISA

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-Linked Immunosorbent Assay.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "RSV"

See other products for "Clone 6H5/H1"


For research use only. Not intended for any clinical use.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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