Mouse Anti-RUNX1 Monoclonal Antibody (VS7-0425-WR805)
CAT#: VS7-0425-WR805
This product is a mouse IgG antibody that binds to human RUNX1. The antibody can be used for WB, IF, ICC, and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4176/if_selected.jpg
Normal Tissue
Figure 2 High expression in breast
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4176/12282_B_3_4_val_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4176/12382_A_8_8.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (HEL, HMC-1, JURKAT)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000159216-RUNX1
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Specifications
- Immunogen
- Synthetic human RUNX1 peptide
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human RUNX1
- Species Reactivity
- Human
- Molecule Class
- Runt-Related Domain-Containing Family
- Applications
- WB, IF, ICC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Familial platelet disorder with associated myeloid malignancy (FPDMM)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- RUNX1; PEA2-Alpha B; AML1-EVI-1 Fusion Protein; CBF-Alpha-2; EVI-1; Runt-Related Transcription Factor 1; Aml1 Oncogene; PEBP2aB; Oncogene AML-1; PEBP2-Alpha B; Acute Myeloid Leukemia 1 Protein; AML1-EVI-1; SL3-3 Enhancer Factor 1 Alpha B Subunit; AMLCR1;
- Gene ID
- 861
- UniProt ID
- Q01196
- Long Name
- Runt Related Transcription Factor 1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Acetylation; Methylation; Phosphoprotein
- Protein Refseq
- NP_001001890.1
- Function
- Activator; DNA-binding; Repressor
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "RUNX1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2922z | Mouse Anti-RUNX1 Recombinant Antibody (clone 24G12) | WB, ELISA, IF, IHC | Mouse IgG1 |
| MOB-2284CT | Recombinant Mouse anti-Human RUNX1 Monoclonal antibody (FQ2566Z) | ICC, IF, WB, IHC-P, IHC-Fr | |
| ZG-078R | Mouse Anti-RUNX1 Recombinant Antibody (ZG-078R) | WB, ELISA | Mouse IgG |
| VS3-WK840 | Rabbit Anti-RUNX1 Recombinant Antibody (VS3-WK840) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS7-0425-WR803 | Mouse Anti-RUNX1 Recombinant Antibody (clone 6C12A5) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3142 | Hi-Affi™ Recombinant Rabbit Anti-RUNX1 Monoclonal Antibody (DS3142AB) | WB, IHC-P | IgG |
| MOR-4728 | Hi-Affi™ Recombinant Rabbit Anti-RUNX1 Monoclonal Antibody (TH242DS) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0199 | APC-A*02:01/Human EVI-1 (MILDEFYNV) MHC Tetramer | FCM | |
| MHC-CN0200 | PE-A*02:01/Human EVI-1 (MILDEFYNV) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6272 | Anti-RUNX1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6273 | Anti-Mouse RUNX1 Immunohistochemistry Kit | IHC |
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