Rabbit Anti-S100A9 Recombinant Antibody (clone 2E9)
CAT#: ZG-0556U
Rabbit Anti-S100A9 Antibody (clone 2E9) displayed high specificity against the S100A9 protein of Human.
Tested Data
Gene Expression
IHC
Figure 1 Rabbit Anti-S100A9 Antibody (ZG-0556U) in IHC
IHC image of ZG-0556U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/33_B12_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_8_5.jpg
Normal Tissue
Figure 3 Esophagus
Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_6_1.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_A_9_3.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (SK-BR-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163220-S100A9
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Specifications
- Immunogen
- A synthesized peptide derived from human S100A9
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human S100A9
- Species Reactivity
- Human
- Clone
- 2E9
- Applications
- ELISA, IHC
- Conjugate
- Unconjugated
- Related Disease
- Cystic Fibrosis and Juvenile Rheumatoid Arthritis.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry. Specific conditions should be optimized by the end user.
IHC:1:50-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "S100A9"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3159 | Hi-Affi™ Recombinant Rabbit Anti-S100A9 Monoclonal Antibody (DS3159AB) | ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0323-CN-S(P) | Mouse Anti-S100A9 Recombinant Antibody (clone IID4B10); scFv Fragment | WB, ELISA, IHC | Mouse scFv |
| HPAB-2539LY-S(P) | Mouse Anti-S100A9 Recombinant Antibody; scFv Fragment (HPAB-2539LY-S(P)) | ELISA, Block | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0323-CN-F(E) | Mouse Anti-S100A9 Recombinant Antibody (clone IID4B10); Fab Fragment | WB, ELISA, IHC | Mouse Fab |
| HPAB-2539LY-F(E) | Mouse Anti-S100A9 Recombinant Antibody; Fab Fragment (HPAB-2539LY-F(E)) | ELISA, Block | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1359-CN | Recombinant Rabbit Anti-S100A9 Monoclonal Antibody (JF096-8) | WB, IF, IHC | Rabbit IgG |
| VS3-FY1299 | Recombinant Rabbit Anti-S100A9 Antibody (clone R05-3C3) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS3-WK1926 | Recombinant Rabbit Anti-S100A9 Capture Antibody (clone R19-5C-9) | ELISA | Rabbit IgG |
| VS3-WK1927 | Recombinant Rabbit Anti-S100A9 Detection Antibody (clone R31-4D-5) | ELISA | Rabbit IgG |
| VS13-YC1030 | Rabbit Anti-S100A9 Recombinant Antibody (VS13-YC1030) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1995 | Anti-S100A9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6317 | Anti-Mouse S100A9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6318 | Anti-Rat S100A9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6316 | Anti-Human S100A9 Immunohistochemistry Kit | IHC |
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