Mouse Anti-S100A9 Recombinant Antibody (clone 3A1H2) (CAT#: VS7-0425-WR813)
This product is a mouse IgG1 antibody that binds to human S100A9. The antibody 3A1H2 recognizes specific amino acid domain of human S100A9 (AA: 1-114). It can be used for IHC and FC assays.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/33_B12_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/33_B12_2_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_8_5.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_8_5.jpg
Figure 3 Esophagus
(Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_6_1.jpg
(Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_6_1.jpg
Figure 4 Colon
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_A_9_3.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_A_9_3.jpg
Figure 5 RNA cell line category: Cell line enriched (SK-BR-3)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163220-S100A9
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163220-S100A9
Specifications
- Immunogen
- Recombinant human S100A9 (AA: 1-114) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human S100A9
- Species Reactivity
- Human
- Clone
- 3A1H2
- Molecule Class
- EF-Hand Calcium-Binding Protein Family
- Applications
- IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- S100A9; Migration Inhibitory Factor-Related Protein 14; L1Ag; CFAG; CAGB; Calgranulin B; MRP14; S100 Calcium Binding Protein A9; MIF; Protein S100-A9; LIAG; Leukocyte L1 Complex Heavy Chain; MRP-14; S100 Calcium-Binding Protein A9; S100 Calcium Binding Pr
- Gene ID
- 6280
- UniProt ID
- P06702
- Long Name
- S100 Calcium Binding Protein A9
- Cellular Localization
- Cell membrane; Cytoplasm; Cytoskeleton; Membrane; Secreted
- Post Translation Modifications
- Methylation; Phosphoprotein; S-nitrosylation
- Protein Refseq
- NP_002956.1
- Function
- Antimicrobial; Antioxidant
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "S100A9"
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Please select product type
IgG Antibody Products
Rabbit Monoclonal Antibody Products
ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1452MZ | Recombinant Mouse Anti-Human S100A9 Antibody (clone OP.245) | ELISA, sELISA | Mouse antibody |
| HPAB-0323-CN | Mouse Anti-S100A9 Recombinant Antibody (clone IID4B10) | WB, ELISA, IHC | Mouse IgG1, κ |
| MRO-1359-CN | Recombinant Rabbit Anti-S100A9 Monoclonal Antibody (JF096-8) | WB, IF, IHC | Rabbit IgG |
| VS3-WK1926 | Recombinant Rabbit Anti-S100A9 Capture Antibody (clone R19-5C-9) | ELISA | Rabbit IgG |
| VS3-WK1927 | Recombinant Rabbit Anti-S100A9 Detection Antibody (clone R31-4D-5) | ELISA | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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