Mouse Anti-SATB2 Recombinant Antibody (clone 7B2C4) (CAT#: VS7-0425-WR829)
This product is a mouse IgG1 antibody that binds to human SATB2. The antibody 7B2C4 recognizes specific amino acid domain of human SATB2 (AA: 377-499). It can be used for IHC and FC assays.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29543/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29543/if_selected.jpg
Figure 2 Cerebral cortex
(Neuronal cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67751/152042_B_9_5.jpg
(Neuronal cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67751/152042_B_9_5.jpg
Figure 3 Colon
(Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67751/152042_A_9_3.jpg
(Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67751/152042_A_9_3.jpg
Figure 4 Rectum
(Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67751/152042_A_4_3.jpg
(Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67751/152042_A_4_3.jpg
Figure 5 Kidney
(Proximal tubules (cell body)
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67751/152042_A_8_5.jpg
(Proximal tubules (cell body)
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67751/152042_A_8_5.jpg
Figure 6 RNA cell line category: Cell line enhanced (HEL, K-562, THP-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000119042-SATB2/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000119042-SATB2/subcellular
Specifications
- Immunogen
- Recombinant human SATB2 (AA: 377-499) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SATB2
- Species Reactivity
- Human
- Clone
- 7B2C4
- Molecule Class
- Dna-Binding Protein
- Applications
- IHC, FC
- Conjugate
- Unconjugated
- Related Disease
- Cleft palate isolated (CPI)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- SATB2; SATB homeobox 2; GLSS; DNA-binding protein SATB2; SATB family member 2; special AT-rich sequence-binding protein 2
- Gene ID
- 23314
- UniProt ID
- A0A024R3U6
- Long Name
- SATB homeobox 2
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001165980.1
- Function
- Chromatin regulator; Developmental protein; DNA-binding; Repressor
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-888 | Recombinant Anti-human SATB2 Antibody | WB, ELISA, IHC, FuncS | IgG |
| MHH-888 | Recombinant Human Anti-human SATB2 Antibody | IF, FC, Biosensors, FuncS | IgG |
| MOB-2453CT | Recombinant Mouse anti-Human SATB2 Monoclonal antibody (nBcdbn) | IP, ICC/IF, IHC-FoFr, WB, IHC-Fr, IHC-P | |
| MRO-1366-CN | Recombinant Rabbit Anti-SATB2 Monoclonal Antibody (JM52-44) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX1000 | Mouse Anti-SATB2 Recombinant Antibody (clone 4G03) | WB, IP, IF | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
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