Mouse Anti-SATB2 Recombinant Antibody (clone 7B2E11)
CAT#: VS7-0425-WR828
This product is a mouse IgG1 antibody that binds to human SATB2. The antibody 7B2E11 recognizes specific amino acid domain of human SATB2 (AA: 377-499). It can be used for IHC and FC assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29543/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67751/152042_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67751/152042_A_9_3.jpg
Normal Tissue
Figure 4 Rectum
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67751/152042_A_4_3.jpg
Normal Tissue
Figure 5 Kidney
Proximal tubules (cell body)
Staining: Low
Intensity: Moderate
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67751/152042_A_8_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HEL, K-562, THP-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000119042-SATB2/subcellular
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Specifications
- Immunogen
- Recombinant human SATB2 (AA: 377-499) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SATB2
- Species Reactivity
- Human
- Clone
- 7B2E11
- Molecule Class
- Dna-Binding Protein
- Applications
- IHC, FC
- Conjugate
- Unconjugated
- Related Disease
- Cleft palate isolated (CPI)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- SATB2; SATB homeobox 2; GLSS; DNA-binding protein SATB2; SATB family member 2; special AT-rich sequence-binding protein 2
- Gene ID
- 23314
- UniProt ID
- A0A024R3U6
- Long Name
- SATB homeobox 2
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001165980.1
- Function
- Chromatin regulator; Developmental protein; DNA-binding; Repressor
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "SATB2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-888 | Recombinant Anti-human SATB2 Antibody | WB, ELISA, IHC, FuncS | IgG |
| MHH-888 | Recombinant Human Anti-human SATB2 Antibody | IF, FC, Biosensors, FuncS | IgG |
| MOB-2453CT | Recombinant Mouse anti-Human SATB2 Monoclonal antibody (nBcdbn) | IP, ICC, IF, IHC-FoFr, WB, IHC-Fr, IHC-P | |
| VS3-QX1000 | Mouse Anti-SATB2 Recombinant Antibody (clone 4G03) | WB, IP, IF | Mouse IgG1 |
| VS3-XY3494 | Rabbit Anti-SATB2 Recombinant Antibody (clone R06-6D8) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-888-F(E) | Recombinant Anti-human SATB2 Antibody Fab Fragment | ELISA, WB, FuncS | Fab |
| MHH-888-F(E) | Recombinant Human Anti-human SATB2 Antibody Fab Fragment | IP, Neut, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-888-S(P) | Recombinant Anti-human SATB2 Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| MHH-888-S(P) | Recombinant Human Anti-human SATB2 Antibody scFv Fragment | WB, FC, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3175 | Hi-Affi™ Recombinant Rabbit Anti-SATB2 Monoclonal Antibody (DS3175AB) | WB, IP, IHC-P, ICC, IF, IHC-Fr | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1038 | CytoStream™ Rabbit Anti-SATB2 Recombinant Antibody (VS13-YC1038) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6356 | Anti-SATB2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6357 | Anti-Mouse SATB2 Immunohistochemistry Kit | IHC |
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