Mouse Anti-SDHA Recombinant Antibody (clone 2A10F6) (CAT#: VS7-0425-WR835)
This product is a mouse IgG2a antibody that binds to human SDHA. The antibody 2A10F6 recognizes specific amino acid domain of human SDHA (AA: 516-665). It can be used for WB, ICC, and FC assays. It cross reacts with mouse.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/if_selected.jpg
Figure 2 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoli & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64582/if_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoli & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64582/if_selected.jpg
Figure 3 IHC staining of human stomach, upper
(Immunohistochemical staining of human stomach, upper shows strong granular cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/ihc_selected.jpg
(Immunohistochemical staining of human stomach, upper shows strong granular cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/ihc_selected.jpg
Figure 4 Testis
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_6_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_6_6.jpg
Figure 5 Heart muscle
(Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_B_4_6.jpg
(Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_B_4_6.jpg
Figure 6 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_7_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_7_8.jpg
Figure 7 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_3.jpg
Figure 8 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_4.jpg
Figure 9 Kidney
(Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_5.jpg
(Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_5.jpg
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073578-SDHA
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073578-SDHA
Specifications
- Immunogen
- Recombinant human SDHA (AA: 516-665) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human SDHA
- Species Reactivity
- Human, Mouse
- Clone
- 2A10F6
- Molecule Class
- Succinate Dehydrogenase
- Applications
- WB, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Mitochondrial complex II deficiency, nuclear type 1 (MC2DN1); Leigh syndrome (LS); Cardiomyopathy, dilated, 1GG (CMD1GG); Pheochromocytoma/paraganglioma syndrome 5 (PPGL5); Neurodegeneration with ataxia and late-onset optic atrophy (NDAXOA)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- FP; PGL5; SDH1; SDH2; SDHF; PPGL5; CMD1GG; MC2DN1; NDAXOA
- Gene ID
- 6389
- UniProt ID
- P31040
- Long Name
- Succinate Dehydrogenase Complex Flavoprotein Subunit A
- Cellular Localization
- Membrane; Mitochondrion; Mitochondrion inner membrane
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_001281261.1
- Function
- Oxidoreductase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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