Mouse Anti-SDHA Recombinant Antibody (clone 2A10F6)
CAT#: VS7-0425-WR835
This product is a mouse IgG2a antibody that binds to human SDHA. The antibody 2A10F6 recognizes specific amino acid domain of human SDHA (AA: 516-665). It can be used for WB, ICC, and FC assays. It cross reacts with mouse.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoli & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64582/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach, upper
Immunohistochemical staining of human stomach, upper shows strong granular cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/ihc_selected.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_6_6.jpg
Normal Tissue
Figure 5 Heart muscle
Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_B_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_7_8.jpg
Normal Tissue
Figure 7 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_4.jpg
Normal Tissue
Figure 9 Kidney
Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_5.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073578-SDHA
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Specifications
- Immunogen
- Recombinant human SDHA (AA: 516-665) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human SDHA
- Species Reactivity
- Human, Mouse
- Clone
- 2A10F6
- Molecule Class
- Succinate Dehydrogenase
- Applications
- WB, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Mitochondrial complex II deficiency, nuclear type 1 (MC2DN1); Leigh syndrome (LS); Cardiomyopathy, dilated, 1GG (CMD1GG); Pheochromocytoma/paraganglioma syndrome 5 (PPGL5); Neurodegeneration with ataxia and late-onset optic atrophy (NDAXOA)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- FP; PGL5; SDH1; SDH2; SDHF; PPGL5; CMD1GG; MC2DN1; NDAXOA
- Gene ID
- 6389
- UniProt ID
- P31040
- Long Name
- Succinate Dehydrogenase Complex Flavoprotein Subunit A
- Cellular Localization
- Membrane; Mitochondrion; Mitochondrion inner membrane
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_001281261.1
- Function
- Oxidoreductase
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Reconstitution Buffer
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