Mouse Anti-SDHA Recombinant Antibody (clone 28G3)
CAT#: MOB-1595z
This product is a mouse antibody that recognizes human SDHA. It can be used in the following applications: WB, ELISA, IF, IHC.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoli & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64582/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach, upper
Immunohistochemical staining of human stomach, upper shows strong granular cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/ihc_selected.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_6_6.jpg
Normal Tissue
Figure 5 Heart muscle
Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_B_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_7_8.jpg
Normal Tissue
Figure 7 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_4.jpg
Normal Tissue
Figure 9 Kidney
Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_5.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073578-SDHA
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Specifications
- Immunogen
- Human Succinate Dehydrogenase Complex, Subunit A, Flavoprotein (Fp)
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Antibody Isotype
- IgG1
- Specificity
- Human SDHA
- Species Reactivity
- Human
- Clone
- 28G3
- Applications
- WB, ELISA, IF, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry.
Target
- Alternative Names
- SDHA; SDH1; Flavoprotein Subunit Of Complex II; SDHF; Succinate Dehydrogenase Complex Flavoprotein Subunit; CMD1GG; Succinate Dehydrogenase [Ubiquinone] Flavoprotein Subunit, Mitochondrial; SDH2; PGL5; EC 1.3.5.1; Succinate Dehydrogenase Complex, Subunit
- UniProt ID
- P31040
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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