Mouse Anti-SEZ6 Recombinant Antibody (HPAB-AP167-YC) (CAT#: HPAB-AP167-YC)
This product is a recombinant mouse antibody that recognizes SEZ6. The antibody was purified by affinity chromatography. It can be used for ELISA, FC and other applications.
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SDS-PAGE
Figure 1 Mouse Anti-SEZ6 Antibody (HPAB-AP167-YC) in SDS-PAGE
SDS-PAGE analysis of HPAB-AP167-YC in non-reduced (Lane 2) and reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
HPLC
Figure 2 Mouse Anti-SEZ6 Antibody (HPAB-AP167-YC) in HPLC
The purity of this antibody is> 95% as determined by SEC-HPLC.
WB
Figure 3 Mouse Anti-SEZ6 Antibody (HPAB-AP167-YC) in WB
Western blot analysis of HPAB-AP167-YC was performed by loading SEZ6 Protein, Human, Recombinant (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-AP167-YC and Goat Anti-Mouse IgG-HRP as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reducing antigen (0.3 μg)
Lane 2: Reducing antigen (0.6 μg)
ELISA
Figure 4 Mouse Anti-SEZ6 Antibody (HPAB-AP167-YC-Biotin) in ELISA
ELISA analysis of HPAB-AP167-YC was performed by coating with SEZ6 Protein, Human, Recombinant (His Tag). Then blocked with BSA, and incubated with Anti-Human SEZ6 Antibody (HPAB-AP167-YC-Biotin). The SA-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
DB
Figure 5 Mouse Anti-SEZ6 Antibody (HPAB-AP167-YC) in DB
Dot Blot analysis of HPAB-AP167-YC was performed by coating with SEZ6 Protein, Human, Recombinant (His Tag).
HPAB-AP167-YC incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000)
Specifications
- Immunogen
- Human SEZ6-Fc
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human SEZ6
- Species Reactivity
- Human, Cynomolgus, Rat
- Applications
- ELISA, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Seizure Related 6 Homolog; Seizure Related 6 Homolog (Mouse); Seizure Protein 6 Homolog; HSEZ-6; BSRPC; SEZ-6
- Gene ID
- 124925
- UniProt ID
- Q53EL9
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Single Domain Antibody Products
Mouse Antibody Products
Humanized Antibody Products
IgG Antibody Products
ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-2594-sdAb | Recombinant Anti-Human SEZ6 VHH Single Domain Antibody | IP, FC, ChiP, Neut | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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