Rabbit Anti-SF3B1 Recombinant Antibody (clone 6C7) (CAT#: ZG-0045U)
The purified Rabbit Anti-SF3B1 Recombinant Antibody (clone 6C7) displayed high specificity against the SF3B1 protein of human and mouse.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-SF3B1 Antibody (ZG-0045U) in WB
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, U-87 whole cell lysate, Mouse Brain whole cell lysate. All lanes: SF3B1 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 146, 17 kDa
Observed band size: 130 kDa
IHC
Figure 2 Rabbit Anti-SF3B1 Antibody (ZG-0045U) in IHC
IHC image of ZG-0045U diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 3 Rabbit Anti-SF3B1 Antibody (ZG-0045U) in IF
Immunofluorescence staining of Hela Cells with ZG-0045U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human SF3B1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SF3B1
- Species Reactivity
- Human, Mouse
- Clone
- 6C7
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Myelodysplastic Syndrome and Autosomal Recessive Pyridoxine-Refractory Sideroblastic Anemia 2
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- Splicing factor 3B subunit 1; Pre-mRNA-splicing factor SF3b 155 kDa subunit; SF3b155; Spliceosome-associated protein 155; SAP 155; SF3B1; SAP155
- Gene ID
- 23451
- UniProt ID
- O75533
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "SF3B1"
Select a product category from the dropdown menu below to view related products.
Please select product type
Rabbit Monoclonal Antibody Products
IgG Antibody Products
Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3240 | Hi-Affi™ Recombinant Rabbit Anti-SF3B1 Monoclonal Antibody (DS3240AB) | WB | IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for ZG-0045U. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: WB, ELISA, IP, FC, FuncS, Neut, IF
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
-1.png)
Application: ELISA
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
