Rabbit Anti-SF3B1 Recombinant Antibody (VS3-FY2871)
CAT#: VS3-FY2871
This anti-SF3B1 antibody is a rabbit recombinant monoclonal antibody. It has been specifically designed to target the human SF3B1 protein. It cross-reacts with mouse, hamster, rat species. The monoclonal antibody can be used in the area of Myelodysplastic Syndrome and Acquired Idiopathic Sideroblastic Anemia.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human salivary gland
Immunohistochemical staining of human salivary gland shows strong nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50275/115976_B_2_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50275/115976_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50275/115976_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50275/115976_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50275/115976_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50275/115976_A_4_6.jpg
Normal Tissue
Figure 7 Skin
Keratinocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50275/115976_B_7_1.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50275/115976_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115524-SF3B1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Hamster, Rat SF3B1
- Species Reactivity
- Human, Mouse, Rat, Hamster
- Applications
- Western Blot, Immunohistochemistry, Immunocytochemistry
- Conjugate
- Unconjugated, FITC
- Related Disease
- Myelodysplastic Syndrome and Acquired Idiopathic Sideroblastic Anemia
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunocytochemistry: 1/50-1/200
Target
- Alternative Names
- Splicing Factor 3b Subunit 1; Splicing Factor 3b, Subunit 1, 155kDa; Spliceosome-Associated Protein 155; SF3b155; SAP155; Pre-MRNA Splicing Factor SF3b, 155 KDa Subunit; Pre-MRNA-Splicing Factor SF3b 155 KDa Subunit; Splicing Factor 3b, Subunit 1, 155kD
- Gene ID
- 23451
- UniProt ID
- O75533
- Sequence Similarities
- Belongs to the SF3B1 family.
- Cellular Localization
- Nucleus, Nucleus speckle
- Post Translation Modifications
- Phosphorylated.
Phosphorylation occurs concomitantly with the splicing catalytic steps.
Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis.
Citrullinated by PADI4. (O75533-SF3B1_HUMAN)
Ubiquitination at Lys182, Lys195, Lys793, Lys816, Lys926, Lys1014, Lys1102, and Lys1292 (NX_O75533 [NX_O75533-1])
Modification sites at PhosphoSitePlus (O75533)
Glycosylation from GlyGen (O75533) 4 sites, 1 O-linked glycan (4 sites)
- Protein Refseq
- XP_047299794.1; XP_047299795.1; XP_047299796.1; XP_047299797.1; XP_054197183.1
- Function
- May also be involved in the assembly of the 'E' complex (PubMed:10882114).
Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SF3B1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3240 | Hi-Affi™ Recombinant Rabbit Anti-SF3B1 Monoclonal Antibody (DS3240AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1382-CN | Recombinant Rabbit Anti-SF3B1 Monoclonal Antibody (JB40-32) | WB, IHC, IF, FC | Rabbit IgG |
| ZG-0045U | Rabbit Anti-SF3B1 Recombinant Antibody (clone 6C7) | ELISA, WB, IHC | Rabbit IgG |
| VS3-WK296 | Rabbit Anti-SF3B1 Recombinant Antibody (clone JB40-32) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1051 | CytoStream™ Rabbit Anti-SF3B1 Recombinant Antibody (VS13-YC1051) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2046 | Anti-SF3B1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6521 | Anti-Mouse SF3B1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6520 | Anti-Human SF3B1 Immunohistochemistry Kit | IHC |
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