CytoStream™ Rabbit Anti-SF3B1 Recombinant Antibody (VS13-YC1051)
CAT#: VS13-YC1051
The CytoStream™ Rabbit Anti-SF3B1 Recombinant Antibody is specific for human, mouse and rat SF3B1. SF3B1 encodes a splicing factor part of U2 snRNP, essential for pre-mRNA splicing, with variants found in hematological disorders. The anti-SF3B1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Normal Tissue
Figure 1 IHC staining of human salivary gland
Immunohistochemical staining of human salivary gland shows strong nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50275/115976_B_2_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50275/115976_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50275/115976_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50275/115976_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50275/115976_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50275/115976_A_4_6.jpg
Normal Tissue
Figure 7 Skin
Keratinocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50275/115976_B_7_1.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50275/115976_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115524-SF3B1
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Specifications
- Immunogen
- Recombinant human SF3B1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SF3B1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 150 mM NaCl and 50% Glycerol.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Splicing Factor 3b Subunit 1; Splicing Factor 3b, Subunit 1, 155kDa; Spliceosome-Associated Protein 155; SF3b155; SAP155; Pre-MRNA Splicing Factor SF3b, 155 KDa Subunit; Pre-MRNA-Splicing Factor SF3b 155 KDa Subunit; Splicing Factor 3b, Subunit 1, 155kD;
- Gene ID
- 23451
- UniProt ID
- O75533
- Long Name
- Splicing Factor 3b Subunit 1
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SF3B1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3240 | Hi-Affi™ Recombinant Rabbit Anti-SF3B1 Monoclonal Antibody (DS3240AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1382-CN | Recombinant Rabbit Anti-SF3B1 Monoclonal Antibody (JB40-32) | WB, IHC, IF, FC | Rabbit IgG |
| ZG-0045U | Rabbit Anti-SF3B1 Recombinant Antibody (clone 6C7) | ELISA, WB, IHC | Rabbit IgG |
| VS3-WK296 | Rabbit Anti-SF3B1 Recombinant Antibody (clone JB40-32) | WB, ELISA | Rabbit IgG |
| VS3-FY2871 | Rabbit Anti-SF3B1 Recombinant Antibody (VS3-FY2871) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2046 | Anti-SF3B1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6521 | Anti-Mouse SF3B1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6520 | Anti-Human SF3B1 Immunohistochemistry Kit | IHC |
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