Mouse Anti-SIGLEC9 Recombinant Antibody (HPAB-M0648-YC)
CAT#: HPAB-M0648-YC
This product is a recombinant mouse antibody that recognizes SIGLEC9. The extracellular domain of human Siglec-7 Fc and Siglec-9 with Fc fragment was used as an immunogen for the mAb generation. It can be used for FC, Cyt, Neut and other applications.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-SIGLEC9 Recombinant Antibody (HPAB-M0648-YC) in WB
Western blot analysis of HPAB-M0648-YC was performed with Recombinant human Siglec-9/CD329 Protein.
HPAB-M0648-YC incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)
ELISA
Figure 2 Mouse Anti-SIGLEC9 Recombinant Antibody (HPAB-M0648-YC) in ELISA
ELISA analysis of HPAB-M0648-YC was performed by coating with Recombinant human Siglec-9/CD329 Protein.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
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Normal Tissue
Figure 1 IHC staining of human spleen
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10682/26903_B_8_4_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10682/26903_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10682/26903_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10682/26903_A_8_4.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10682/26903_A_6_6.jpg
Normal Tissue
Figure 6 Spleen
Cells in red pulp Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10682/26903_B_8_4.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (Karpas-707, RPMI-8226)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000129450-SIGLEC9
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Specifications
- Immunogen
- The extracellular domain of human Siglec-7 Fc and Siglec-9 with Fc fragment
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human SIGLEC9
- Species Reactivity
- Human
- Applications
- FC, Cyt, Neut
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
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Effective For Cytotoxicity Assays
We employed this recombinant mouse antibody in cytotoxicity assays involving SIGLEC9-expressing cells. The antibody functioned as expected, likely due to its specific recognition of the extracellular domain. The purity was high, and we appreciated the detailed information provided regarding the immunogen used for generation.
Clean Flow Cytometry Profiles
We used this antibody for Flow Cytometry to phenotype immune cells. The staining was robust and allowed us to clearly identify SIGLEC9 positive populations. It is a reliable reagent for investigating sialic acid-binding immunoglobulin-like lectins, and the recombinant nature ensures reproducibility for our longitudinal studies.
Q&As
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What was used as the immunogen for this antibody?
A: The immunogen used to generate this antibody was the extracellular domain of human Siglec-7 Fc and Siglec-9 with an Fc fragment. This strategy ensures the antibody recognizes the accessible extracellular regions of the SIGLEC9 protein, which is critical for flow cytometry and functional assays.
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What specific applications is this antibody recommended for?
A: This Mouse Anti-SIGLEC9 Recombinant Antibody is recommended for Flow Cytometry (FC), Cytotoxicity assays (Cyt), and Neutralization (Neut). These applications allow for both the phenotypic characterization of cells and the investigation of the functional role of Siglec-9 in immune responses.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1963CQ | Recombinant Mouse Anti-SIGLEC9 Antibody (CBL273) | FC, ELISA, WB, Neut | IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1964CQ | Recombinant Mouse Anti-SIGLEC9 Antibody (MM0552-6K12) | WB, Neut | IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1965CQ | Recombinant Mouse Anti-SIGLEC9 Antibody (CBL164) | Neut, WB | IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1966CQ | Recombinant Mouse Anti-SIGLEC9 Antibody (CBL711) | WB, FC, CyTOF, ELISA(Cap), Neut | IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0649-YC | Mouse Anti-SIGLEC9 Recombinant Antibody (HPAB-M0649-YC) | FC, Cyt, Neut | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0650-YC | Mouse Anti-SIGLEC9 Recombinant Antibody (HPAB-M0650-YC) | FC, Cyt, Neut | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0651-YC | Mouse Anti-SIGLEC9 Recombinant Antibody (HPAB-M0651-YC) | FC, Cyt | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0648-YC-S(P) | Mouse Anti-SIGLEC9 Recombinant Antibody; scFv Fragment (HPAB-M0648-YC-S(P)) | FC, Cyt, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0649-YC-S(P) | Mouse Anti-SIGLEC9 Recombinant Antibody; scFv Fragment (HPAB-M0649-YC-S(P)) | FC, Cyt, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0650-YC-S(P) | Mouse Anti-SIGLEC9 Recombinant Antibody; scFv Fragment (HPAB-M0650-YC-S(P)) | FC, Cyt, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0651-YC-S(P) | Mouse Anti-SIGLEC9 Recombinant Antibody; scFv Fragment (HPAB-M0651-YC-S(P)) | FC, Cyt | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0648-YC-F(E) | Mouse Anti-SIGLEC9 Recombinant Antibody; Fab Fragment (HPAB-M0648-YC-F(E)) | FC, Cyt, Neut | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0649-YC-F(E) | Mouse Anti-SIGLEC9 Recombinant Antibody; Fab Fragment (HPAB-M0649-YC-F(E)) | FC, Cyt, Neut | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0650-YC-F(E) | Mouse Anti-SIGLEC9 Recombinant Antibody; Fab Fragment (HPAB-M0650-YC-F(E)) | FC, Cyt, Neut | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0651-YC-F(E) | Mouse Anti-SIGLEC9 Recombinant Antibody; Fab Fragment (HPAB-M0651-YC-F(E)) | FC, Cyt | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-3335LY | Human Anti-SIGLEC9 Recombinant Antibody (HPAB-3335LY) | FC | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-3336LY | Human Anti-SIGLEC9 Recombinant Antibody (HPAB-3336LY) | FC | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-3335LY-S(P) | Human Anti-SIGLEC9 Recombinant Antibody; scFv Fragment (HPAB-3335LY-S(P)) | FC | Humanized scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-3335LY-F(E) | Human Anti-SIGLEC9 Recombinant Antibody; Fab Fragment (HPAB-3335LY-F(E)) | FC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY479 | Mouse Anti-NHP SIGLEC9 Recombinant Antibody (clone OTI6D8) | WB, IHC, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC471 | Recombinant Anti-SIGLEC9 Vesicular Antibody, EV Displayed (VS-0425-YC471) | Cyt, ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6588 | Anti-SIGLEC9 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6589 | Anti-Human SIGLEC9 Immunohistochemistry Kit | IHC |
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