What was used as the immunogen for this antibody?

The immunogen used to generate this antibody was the extracellular domain of human Siglec-7 Fc and Siglec-9 with an Fc fragment. This strategy ensures the antibody recognizes the accessible extracellular regions of the SIGLEC9 protein, which is critical for flow cytometry and functional assays.

What specific applications is this antibody recommended for?

This Mouse Anti-SIGLEC9 Recombinant Antibody is recommended for Flow Cytometry (FC), Cytotoxicity assays (Cyt), and Neutralization (Neut). These applications allow for both the phenotypic characterization of cells and the investigation of the functional role of Siglec-9 in immune responses.

Mouse Anti-SIGLEC9 Recombinant Antibody (HPAB-M0648-YC)

CAT#: HPAB-M0648-YC

This product is a recombinant mouse antibody that recognizes SIGLEC9. The extracellular domain of human Siglec-7 Fc and Siglec-9 with Fc fragment was used as an immunogen for the mAb generation. It can be used for FC, Cyt, Neut and other applications.

Tested Data Gene Expression

Figure 1 Mouse Anti-SIGLEC9 Recombinant Antibody (HPAB-M0648-YC) in WB

Western blot analysis of HPAB-M0648-YC was performed with Recombinant human Siglec-9/CD329 Protein.
HPAB-M0648-YC incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)

ELISA

Figure 2 Mouse Anti-SIGLEC9 Recombinant Antibody (HPAB-M0648-YC) in ELISA

ELISA analysis of HPAB-M0648-YC was performed by coating with Recombinant human Siglec-9/CD329 Protein.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)

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Normal Tissue Figure 1 IHC staining of human spleen

Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10682/26903_B_8_4_selected.jpg

Normal Tissue Figure 2 Cerebral cortex

Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10682/26903_B_7_5.jpg

Normal Tissue Figure 3 Colon

Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10682/26903_A_9_3.jpg

Normal Tissue Figure 4 Liver

Cholangiocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10682/26903_A_8_4.jpg

Normal Tissue Figure 5 Testis

Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10682/26903_A_6_6.jpg

Normal Tissue Figure 6 Spleen

Cells in red pulp Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10682/26903_B_8_4.jpg

RNA Expression Figure 7 RNA cell line category: Group enriched (Karpas-707, RPMI-8226)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000129450-SIGLEC9

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Specifications

Immunogen
The extracellular domain of human Siglec-7 Fc and Siglec-9 with Fc fragment

Host Species
Mouse

Type
Mouse IgG

Specificity
Human SIGLEC9

Species Reactivity
Human

Applications
FC, Cyt, Neut

Product Property

Purity
>95% as determined by SDS-PAGE

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
Sialic Acid Binding Ig Like Lectin 9; Protein FOAP-9; Siglec-9; CDw329; Sialic Acid Binding Ig-Like Lectin 9; Sialic Acid-Binding Ig-Like Lectin 9;

Gene ID
27180

UniProt ID
Q9Y336

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Breast cancer biomarkers at key points during disease progression

Effective For Cytotoxicity Assays

We employed this recombinant mouse antibody in cytotoxicity assays involving SIGLEC9-expressing cells. The antibody functioned as expected, likely due to its specific recognition of the extracellular domain. The purity was high, and we appreciated the detailed information provided regarding the immunogen used for generation.

Clean Flow Cytometry Profiles

We used this antibody for Flow Cytometry to phenotype immune cells. The staining was robust and allowed us to clearly identify SIGLEC9 positive populations. It is a reliable reagent for investigating sialic acid-binding immunoglobulin-like lectins, and the recombinant nature ensures reproducibility for our longitudinal studies.

Q&As

What was used as the immunogen for this antibody?

A: The immunogen used to generate this antibody was the extracellular domain of human Siglec-7 Fc and Siglec-9 with an Fc fragment. This strategy ensures the antibody recognizes the accessible extracellular regions of the SIGLEC9 protein, which is critical for flow cytometry and functional assays.
What specific applications is this antibody recommended for?

A: This Mouse Anti-SIGLEC9 Recombinant Antibody is recommended for Flow Cytometry (FC), Cytotoxicity assays (Cyt), and Neutralization (Neut). These applications allow for both the phenotypic characterization of cells and the investigation of the functional role of Siglec-9 in immune responses.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-M0648-YC.pdf

MSDS

MSDS - HPAB-M0648-YC.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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CAT	Product Name	Application	Type
HPAB-M0649-YC	Mouse Anti-SIGLEC9 Recombinant Antibody (HPAB-M0649-YC)	FC, Cyt, Neut	Mouse IgG

CAT	Product Name	Application	Type
HPAB-M0650-YC	Mouse Anti-SIGLEC9 Recombinant Antibody (HPAB-M0650-YC)	FC, Cyt, Neut	Mouse IgG

CAT	Product Name	Application	Type
HPAB-M0651-YC	Mouse Anti-SIGLEC9 Recombinant Antibody (HPAB-M0651-YC)	FC, Cyt	Mouse IgG

CAT	Product Name	Application	Type
HPAB-M0648-YC-S(P)	Mouse Anti-SIGLEC9 Recombinant Antibody; scFv Fragment (HPAB-M0648-YC-S(P))	FC, Cyt, Neut	Mouse scFv

CAT	Product Name	Application	Type
HPAB-M0649-YC-S(P)	Mouse Anti-SIGLEC9 Recombinant Antibody; scFv Fragment (HPAB-M0649-YC-S(P))	FC, Cyt, Neut	Mouse scFv

CAT	Product Name	Application	Type
HPAB-M0650-YC-S(P)	Mouse Anti-SIGLEC9 Recombinant Antibody; scFv Fragment (HPAB-M0650-YC-S(P))	FC, Cyt, Neut	Mouse scFv

CAT	Product Name	Application	Type
HPAB-M0651-YC-S(P)	Mouse Anti-SIGLEC9 Recombinant Antibody; scFv Fragment (HPAB-M0651-YC-S(P))	FC, Cyt	Mouse scFv

CAT	Product Name	Application	Type
HPAB-M0648-YC-F(E)	Mouse Anti-SIGLEC9 Recombinant Antibody; Fab Fragment (HPAB-M0648-YC-F(E))	FC, Cyt, Neut	Mouse Fab

CAT	Product Name	Application	Type
HPAB-M0649-YC-F(E)	Mouse Anti-SIGLEC9 Recombinant Antibody; Fab Fragment (HPAB-M0649-YC-F(E))	FC, Cyt, Neut	Mouse Fab

CAT	Product Name	Application	Type
HPAB-M0650-YC-F(E)	Mouse Anti-SIGLEC9 Recombinant Antibody; Fab Fragment (HPAB-M0650-YC-F(E))	FC, Cyt, Neut	Mouse Fab

CAT	Product Name	Application	Type
HPAB-M0651-YC-F(E)	Mouse Anti-SIGLEC9 Recombinant Antibody; Fab Fragment (HPAB-M0651-YC-F(E))	FC, Cyt	Mouse Fab

CAT	Product Name	Application	Type
HPAB-3335LY	Human Anti-SIGLEC9 Recombinant Antibody (HPAB-3335LY)	FC	Humanized IgG

CAT	Product Name	Application	Type
HPAB-3336LY	Human Anti-SIGLEC9 Recombinant Antibody (HPAB-3336LY)	FC	Humanized IgG

CAT	Product Name	Application	Type
HPAB-3335LY-S(P)	Human Anti-SIGLEC9 Recombinant Antibody; scFv Fragment (HPAB-3335LY-S(P))	FC	Humanized scfv

CAT	Product Name	Application	Type
HPAB-3335LY-F(E)	Human Anti-SIGLEC9 Recombinant Antibody; Fab Fragment (HPAB-3335LY-F(E))	FC	Humanized Fab

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VS-1024-XY479	Mouse Anti-NHP SIGLEC9 Recombinant Antibody (clone OTI6D8)	WB, IHC, ICC	Mouse IgG1

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VS-0425-YC471	Recombinant Anti-SIGLEC9 Vesicular Antibody, EV Displayed (VS-0425-YC471)	Cyt, ELISA, FC, Neut, Cell-uptake

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VS-0525-XY6588	Anti-SIGLEC9 Immunohistochemistry Kit	IHC

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