Mouse Anti-NHP SIGLEC9 Recombinant Antibody (clone OTI6D8)
CAT#: VS-1024-XY479
Designed to target SIGLEC9, this antibody showcases exceptional reactivity with dog, human, and non-human primate species. The immunogen comprises the full-length human recombinant protein of SIGLEC9, ensuring optimal specificity and sensitivity. Its efficacy spans various applications, including Western blotting, immunohistochemistry, and immunocytochemistry. Researchers investigating immunological responses and cellular interactions involving SIGLEC9 will find this antibody an indispensable asset in their experimental toolkit, offering reliable results across multiple biological contexts.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human spleen
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10682/26903_B_8_4_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10682/26903_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10682/26903_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10682/26903_A_8_4.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10682/26903_A_6_6.jpg
Normal Tissue
Figure 6 Spleen
Cells in red pulp Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10682/26903_B_8_4.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (Karpas-707, RPMI-8226)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000129450-SIGLEC9
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Specifications
- Immunogen
- Full length human recombinant protein of SIGLEC9
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- SIGLEC9
- Species Reactivity
- Dog, Human, Non-human primate
- Clone
- OTI6D8
- Applications
- WB, IHC, ICC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:20, IHC: 1:50, ICC: 1:50
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SIGLEC9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1963CQ | Recombinant Mouse Anti-SIGLEC9 Antibody (CBL273) | FC, ELISA, WB, Neut | IgG2a |
| NEUT-1964CQ | Recombinant Mouse Anti-SIGLEC9 Antibody (MM0552-6K12) | WB, Neut | IgG2 |
| NEUT-1965CQ | Recombinant Mouse Anti-SIGLEC9 Antibody (CBL164) | Neut, WB | IgG2 |
| NEUT-1966CQ | Recombinant Mouse Anti-SIGLEC9 Antibody (CBL711) | WB, FC, CyTOF, ELISA(Cap), Neut | IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0648-YC | Mouse Anti-SIGLEC9 Recombinant Antibody (HPAB-M0648-YC) | FC, Cyt, Neut | Mouse IgG |
| HPAB-M0649-YC | Mouse Anti-SIGLEC9 Recombinant Antibody (HPAB-M0649-YC) | FC, Cyt, Neut | Mouse IgG |
| HPAB-M0650-YC | Mouse Anti-SIGLEC9 Recombinant Antibody (HPAB-M0650-YC) | FC, Cyt, Neut | Mouse IgG |
| HPAB-M0651-YC | Mouse Anti-SIGLEC9 Recombinant Antibody (HPAB-M0651-YC) | FC, Cyt | Mouse IgG |
| HPAB-3335LY | Human Anti-SIGLEC9 Recombinant Antibody (HPAB-3335LY) | FC | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0648-YC-S(P) | Mouse Anti-SIGLEC9 Recombinant Antibody; scFv Fragment (HPAB-M0648-YC-S(P)) | FC, Cyt, Neut | Mouse scFv |
| HPAB-M0649-YC-S(P) | Mouse Anti-SIGLEC9 Recombinant Antibody; scFv Fragment (HPAB-M0649-YC-S(P)) | FC, Cyt, Neut | Mouse scFv |
| HPAB-M0650-YC-S(P) | Mouse Anti-SIGLEC9 Recombinant Antibody; scFv Fragment (HPAB-M0650-YC-S(P)) | FC, Cyt, Neut | Mouse scFv |
| HPAB-M0651-YC-S(P) | Mouse Anti-SIGLEC9 Recombinant Antibody; scFv Fragment (HPAB-M0651-YC-S(P)) | FC, Cyt | Mouse scFv |
| HPAB-3335LY-S(P) | Human Anti-SIGLEC9 Recombinant Antibody; scFv Fragment (HPAB-3335LY-S(P)) | FC | Humanized scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0648-YC-F(E) | Mouse Anti-SIGLEC9 Recombinant Antibody; Fab Fragment (HPAB-M0648-YC-F(E)) | FC, Cyt, Neut | Mouse Fab |
| HPAB-M0649-YC-F(E) | Mouse Anti-SIGLEC9 Recombinant Antibody; Fab Fragment (HPAB-M0649-YC-F(E)) | FC, Cyt, Neut | Mouse Fab |
| HPAB-M0650-YC-F(E) | Mouse Anti-SIGLEC9 Recombinant Antibody; Fab Fragment (HPAB-M0650-YC-F(E)) | FC, Cyt, Neut | Mouse Fab |
| HPAB-M0651-YC-F(E) | Mouse Anti-SIGLEC9 Recombinant Antibody; Fab Fragment (HPAB-M0651-YC-F(E)) | FC, Cyt | Mouse Fab |
| HPAB-3335LY-F(E) | Human Anti-SIGLEC9 Recombinant Antibody; Fab Fragment (HPAB-3335LY-F(E)) | FC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC471 | Recombinant Anti-SIGLEC9 Vesicular Antibody, EV Displayed (VS-0425-YC471) | Cyt, ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6588 | Anti-SIGLEC9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6589 | Anti-Human SIGLEC9 Immunohistochemistry Kit | IHC |
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