Rabbit Anti-SIN3A Recombinant Antibody (clone 10G3) (CAT#: ZG-0560U)
Rabbit Anti-SIN3A Antibody (clone 10G3) displayed high specificity against the SIN3A protein of Human.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-SIN3A Antibody (ZG-0560U) in WB
Western Blot
Positive WB detected in: K562 whole cell lysate, MCF-7 whole cell lysate
All lanes: mSin3A antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 146 kDa
Observed band size: 146 kDa
IHC
Figure 2 Rabbit Anti-SIN3A Antibody (ZG-0560U) in IHC
IHC image of ZG-0560U diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 3 Rabbit Anti-SIN3A Antibody (ZG-0560U) in IHC
IHC image of ZG-0560U diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 4 Rabbit Anti-SIN3A Antibody (ZG-0560U) in IF
Immunofluorescence staining of HepG2 Cells with ZG-0560U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human mSin3A
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SIN3A
- Species Reactivity
- Human
- Clone
- 10G3
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Witteveen-Kolk Syndrome and Chromosome 15Q24 Deletion Syndrome.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- Paired amphipathic helix protein Sin3a; Histone deacetylase complex subunit Sin3a; Transcriptional corepressor Sin3a; SIN3A
- Gene ID
- 25942
- UniProt ID
- Q96ST3
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "SIN3A"
Select a product category from the dropdown menu below to view related products.
Please select product type
Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3265 | Hi-Affi™ Recombinant Rabbit Anti-SIN3A Monoclonal Antibody (DS3265AB) | WB, IHC, ICC | IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for ZG-0560U. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, FC, IF, Inhib, ELISA, IHC
Application: Neut, IHC, Activ, FuncS, IF, ELISA
-2.png)
Application: ELISA
Application: FC
Application: ELISA, Activ, Block
For Research Use Only. Not For Clinical Use.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
