Rabbit Anti-SKP2 Recombinant Antibody (clone R02-6V3) (CAT#: VS3-XY3507)
The rabbit recombinant antibody specifically recognizes SKP2 (S-phase kinase associated protein 2), which promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner. It specifically reacts with human PMEL. The antibody specifically reacts with human, mouse and rat SKP2. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin, Flow cytometry and Immunofluorescence is 1/500-1/1000, 1/50-1/100, 1/50-1/100 and 1/50-1/200, respectively.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm, nucleoli & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51196/if_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm, nucleoli & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51196/if_selected.jpg
Figure 2 Colon
(Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13491/144767_A_9_3.jpg
(Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13491/144767_A_9_3.jpg
Figure 3 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13491/144767_A_4_6.jpg
(Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13491/144767_A_4_6.jpg
Figure 4 RNA cell line category: Cell line enhanced (BEWO, RH-30)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145604-SKP2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145604-SKP2
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SKP2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-6V3
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Lung Cancer; Hepatitis C Virus
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- p45; FBL1; FLB1; FBXL1
- Gene ID
- 6502
- UniProt ID
- Q13309
- Long Name
- S-phase kinase associated protein 2
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitinated by the APC/C complex, leading to its degradation by the proteasome.
Deubiquitinated by USP13.
- Protein Refseq
- XP_054209162.1
- Function
- Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "SKP2"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1784MZ | Recombinant Mouse Anti-Human SKP2 Antibody (clone nBcdbn79998) | WB | Mouse antibody |
| ZG-0126U | Rabbit Anti-SKP2 Recombinant Antibody (clone 6D4) | ELISA, IHC, IF | Rabbit IgG |
| ZG-0562U | Rabbit Anti-SKP2 Recombinant Antibody (clone 3C11) | ELISA, IF, FC | Rabbit IgG |
| VS3-QX1016 | Mouse Anti-SKP2 Recombinant Antibody (clone 4D8-B9-F3) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-FY1343 | Recombinant Rabbit Anti-SKP2 Antibody (clone R08-0A2) | WB, ICC, IF | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3275 | Hi-Affi™ Recombinant Rabbit Anti-SKP2 Monoclonal Antibody (DS3275AB) | WB, ICC | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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