Rabbit Anti-SKP2 Recombinant Antibody (clone R02-6V3)
CAT#: VS3-XY3507
The rabbit recombinant antibody specifically recognizes SKP2 (S-phase kinase associated protein 2), which promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner. It specifically reacts with human PMEL. The antibody specifically reacts with human, mouse and rat SKP2. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin, Flow cytometry and Immunofluorescence is 1/500-1/1000, 1/50-1/100, 1/50-1/100 and 1/50-1/200, respectively.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm, nucleoli & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51196/if_selected.jpg
Normal Tissue
Figure 2 Colon
Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13491/144767_A_9_3.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13491/144767_A_4_6.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (BEWO, RH-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145604-SKP2
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SKP2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-6V3
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Lung Cancer; Hepatitis C Virus
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- p45; FBL1; FLB1; FBXL1
- Gene ID
- 6502
- UniProt ID
- Q13309
- Long Name
- S-phase kinase associated protein 2
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitinated by the APC/C complex, leading to its degradation by the proteasome.
Deubiquitinated by USP13.
- Protein Refseq
- XP_054209162.1
- Function
- Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SKP2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1784MZ | Recombinant Mouse Anti-Human SKP2 Antibody (clone nBcdbn79998) | WB | Mouse antibody |
| ZG-091R | Mouse Anti-SKP2 Recombinant Antibody (ZG-091R) | WB, ELISA | Mouse IgG |
| ZG-0126U | Rabbit Anti-SKP2 Recombinant Antibody (clone 6D4) | ELISA, IHC, IF | Rabbit IgG |
| VS3-QX1016 | Mouse Anti-SKP2 Recombinant Antibody (clone 4D8-B9-F3) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS7-0425-WR868 | Mouse Anti-SKP2 Monoclonal Antibody (clone 6G9D10) | WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3275 | Hi-Affi™ Recombinant Rabbit Anti-SKP2 Monoclonal Antibody (DS3275AB) | WB, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY483 | Mouse Anti-NHP SKP2 Recombinant Antibody (clone SKP2-8D9) | WB, IF, IP, ELISA | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2080 | Anti-SKP2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6616 | Anti-Human SKP2 Immunohistochemistry Kit | IHC |
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