Rabbit Anti-SNX5 Recombinant Antibody (clone R06-8H7)
CAT#: VS3-FY1617
Clone R06-8H7 is a rabbit IgG recombinant monoclonal antibody that exhibits high affinity to human SNX5 protein, a calculated weight of 47 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R06-8H7 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunofluorescence (dilution range 1:50-1:200), immunoprecipitation (dilution range 1:20).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/76450/1816_H7_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human thyroid gland
Immunohistochemical staining of human thyroid gland shows moderate cytoplasmiuc positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51187/130224_B_1_5_selected.jpg
Normal Tissue
Figure 3 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20679/ihc_selected.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51187/130224_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51187/130224_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51187/130224_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51187/130224_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (Hep G2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000089006-SNX5
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Specifications
- Immunogen
- Recombinant human SNX5 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SNX5
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-8H7
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 47 kDa, while the observed molecular weight stands at 47 kDa.
- Related Disease
- Fanconi Anemia, Complementation Group A and Parkinson Disease, Late-Onset
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Sorting Nexin 5
- Gene ID
- 27131
- UniProt ID
- Q9Y5X3
- Sequence Similarities
- Belongs to the sorting nexin family.
- Cellular Localization
- Cytoplasmic vesicle
- Protein Refseq
- NP_001269383.1; NP_055241.1; NP_689413.1
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SNX5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1247 | PE-B*44:03/Human SNX5 (EEPTVIKKY) MHC Tetramer | FCM | |
| MHC-LC1248 | APC-B*44:03/Human SNX5 (EEPTVIKKY) MHC Tetramer | FCM | |
| MHC-LC1249 | BV421-B*44:03/Human SNX5 (EEPTVIKKY) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3364 | Hi-Affi™ Recombinant Rabbit Anti-SNX5 Monoclonal Antibody (DS3364AB) | WB, IHC-P, ICC, IF, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2139 | Anti-SNX5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6793 | Anti-Mouse SNX5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6792 | Anti-Human SNX5 Immunohistochemistry Kit | IHC |
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