Mouse Anti-SOCS3 Recombinant Antibody (clone 5E12E7F1)
CAT#: VS7-0425-WR903
This product is a mouse IgG1 antibody that binds to human SOCS3. The antibody 5E12E7F1 recognizes specific amino acid domain of human SOCS3 (AA: 1-225). It can be used for IHC, ICC, and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68569/1358_H11_4_selected.jpg
Normal Tissue
Figure 2 IHC staining of human gall bladder
Immunohistochemical staining of human gall bladder shows distinct cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12220/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12220/33045_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12220/33045_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12220/33045_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12220/33045_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Low Intensity: Weak Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12220/33045_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HDLM-2, HSkMC, U-266/70)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000184557-SOCS3
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Specifications
- Immunogen
- Recombinant human SOCS3 (AA: 1-225) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SOCS3
- Species Reactivity
- Human
- Clone
- 5E12E7F1
- Molecule Class
- Suppressor Of Cytokine Signaling (Socs) Family
- Applications
- IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- SOCS3; SSI3; CIS3; ATOD4; CIS-3; STAT-Induced STAT Inhibitor 3; Suppressor Of Cytokine Signaling 3; Cish3; SSI-3; SOCS-3; Cytokine-Inducible SH2 Protein 3
- Gene ID
- 9021
- UniProt ID
- O14543
- Long Name
- Suppressor of cytokine signaling 3
- Cellular Localization
- cytoplasmic side of plasma membrane; cytosol
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_001365861.1
- Function
- Signal transduction inhibitor
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "SOCS3"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3573z | Mouse Anti-SOCS3 Recombinant Antibody (clone 32G3) | WB, IP, ELISA | Mouse IgG2b, κ |
| ZG-105R | Mouse Anti-SOCS3 Recombinant Antibody (ZG-105R) | WB, ELISA, IHC | Mouse IgG |
| VS3-CJ6 | Mouse Anti-SOCS3 Recombinant Antibody (VS3-CJ6) | WB, ELISA | Mouse IgG |
| VS7-0425-WR902 | Mouse Anti-SOCS3 Recombinant Antibody (clone 3A10A12) | WB, IHC, ICC, FC | Mouse IgG1 |
| VS7-0425-WR904 | Mouse Anti-SOCS3 Recombinant Antibody (VS7-0425-WR904) | WB, IHC-P, IF | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6800 | Anti-SOCS3 Immunohistochemistry Kit | IHC |
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