Rabbit Anti-SOX9 Recombinant Antibody (clone R06-7C6) (CAT#: VS3-FY1631)
The R06-7C6 recombinant monoclonal antibody exhibits high affinity to human SOX9. It cross-reacts with mouse, rat. The specificity of the SOX9 antibody was confirmed by western blot analysis of SOX9 in C6, 3T3, Hela lysates.
Specific Inquiry
WB
Figure 1 Anti-SOX9 Antibody (VS3-FY1631) in WB.
The SOX9 expression in C6, 3T3, Hela lysates were detected using the anti-SOX9 by western blot assay.
Specifications
- Immunogen
- Recombinant human SOX9 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SOX9
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-7C6
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 56 kDa, while the observed molecular weight stands at 70 kDa.
- Related Disease
- Campomelic Dysplasia and 46,Xx Sex Reversal 2
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- SRY-Box 9; Campomelic Dysplasia, Autosomal Sex-Reversal; SRY (Sex-Determining Region Y)-Box 9 Protein; SRY (Sex Determining Region Y)-Box 9; SRY (Sex Determining Region Y)-Box9; SRY-Related HMG-Box, Gene 9; Transcription Factor SOX-9
- Gene ID
- 6662
- UniProt ID
- P48436
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Acetylated; acetylation impairs nuclear localization and ability to transactivate expression of target genes. Deacetylated by SIRT1., Phosphorylation at Ser-64 and Ser-211 by PKA increases transcriptional activity and may help delay chondrocyte maturation downstream of PTHLH/PTHrP signaling. Phosphorylation at either Ser-64 or Ser-211 is required for sumoylation, but phosphorylation is not dependent on sumoylation. Phosphorylated on tyrosine residues; tyrosine dephosphorylation by PTPN11/SHP2 blocks SOX9 phosphorylation by PKA and subsequent SUMOylation., Ubiquitinated; ubiquitination leads to proteasomal degradation and is negatively regulated by DDRGK1., Sumoylated; phosphorylation at either Ser-64 or Ser-211 is required for sumoylation. Sumoylation is induced by BMP signaling pathway.
- Protein Refseq
- NP_000337.1
- Function
- Plays a central role in successive steps of chondrocyte differentiation (By similarity).
Absolutely required for precartilaginous condensation, the first step in chondrogenesis during which skeletal progenitors differentiate into prechondrocytes (By similarity).
Together with SOX5 and SOX6, required for overt chondrogenesis when condensed prechondrocytes differentiate into early stage chondrocytes, the second step in chondrogenesis (By similarity).
Later, required to direct hypertrophic maturation and block osteoblast differentiation of growth plate chondrocytes: maintains chondrocyte columnar proliferation, delays prehypertrophy and then prevents osteoblastic differentiation of chondrocytes by lowering beta-catenin (CTNNB1) signaling and RUNX2 expression (By similarity).
Also required for chondrocyte hypertrophy, both indirectly, by keeping the lineage fate of chondrocytes, and directly, by remaining present in upper hypertrophic cells and transactivating COL10A1 along with MEF2C (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
cAMP Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "SOX9"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
Rabbit Monoclonal Antibody Products
IgM Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3882z | Mouse Anti-SOX9 Recombinant Antibody (clone 15C2) | WB, ICC, IF, ELISA | Mouse IgG1 |
| MOB-1914CT | Recombinant Mouse anti-Human SOX9 Monoclonal antibody (3C10) | ELISA, ICC/IF, IHC-P, WB | |
| ZG-0122U | Rabbit Anti-SOX9 Recombinant Antibody (clone 5H12) | ELISA, IHC | Rabbit IgG |
| VS3-WK1930 | Recombinant Rabbit Anti-SOX9 Capture Antibody (clone R65-8T-3) | ELISA | Rabbit IgG |
| VS3-WK1931 | Recombinant Rabbit Anti-SOX9 Detection Antibody (clone R97-6V-1) | ELISA | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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