Rabbit Anti-SOX9 Recombinant Antibody (clone R06-7C6)
CAT#: VS3-FY1631
The R06-7C6 recombinant monoclonal antibody exhibits high affinity to human SOX9. It cross-reacts with mouse, rat. The specificity of the SOX9 antibody was confirmed by western blot analysis of SOX9 in C6, 3T3, Hela lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22456/if_selected.jpg
Normal Tissue
Figure 2 Salivary gland.
High expression in salivary gland
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_B_1_1_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity: 75%-25% Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_5_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (AN3-CA, Hep G2, OE19, U-251 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125398-SOX9
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WB
Figure 1 Anti-SOX9 Antibody (VS3-FY1631) in WB.
The SOX9 expression in C6, 3T3, Hela lysates were detected using the anti-SOX9 by western blot assay.
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Specifications
- Immunogen
- Recombinant human SOX9 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SOX9
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-7C6
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 56 kDa, while the observed molecular weight stands at 70 kDa.
- Related Disease
- Campomelic Dysplasia and 46,Xx Sex Reversal 2
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- SRY-Box 9; Campomelic Dysplasia, Autosomal Sex-Reversal; SRY (Sex-Determining Region Y)-Box 9 Protein; SRY (Sex Determining Region Y)-Box 9; SRY (Sex Determining Region Y)-Box9; SRY-Related HMG-Box, Gene 9; Transcription Factor SOX-9
- Gene ID
- 6662
- UniProt ID
- P48436
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Acetylated; acetylation impairs nuclear localization and ability to transactivate expression of target genes. Deacetylated by SIRT1., Phosphorylation at Ser-64 and Ser-211 by PKA increases transcriptional activity and may help delay chondrocyte maturation downstream of PTHLH/PTHrP signaling. Phosphorylation at either Ser-64 or Ser-211 is required for sumoylation, but phosphorylation is not dependent on sumoylation. Phosphorylated on tyrosine residues; tyrosine dephosphorylation by PTPN11/SHP2 blocks SOX9 phosphorylation by PKA and subsequent SUMOylation., Ubiquitinated; ubiquitination leads to proteasomal degradation and is negatively regulated by DDRGK1., Sumoylated; phosphorylation at either Ser-64 or Ser-211 is required for sumoylation. Sumoylation is induced by BMP signaling pathway.
- Protein Refseq
- NP_000337.1
- Function
- Plays a central role in successive steps of chondrocyte differentiation (By similarity).
Absolutely required for precartilaginous condensation, the first step in chondrogenesis during which skeletal progenitors differentiate into prechondrocytes (By similarity).
Together with SOX5 and SOX6, required for overt chondrogenesis when condensed prechondrocytes differentiate into early stage chondrocytes, the second step in chondrogenesis (By similarity).
Later, required to direct hypertrophic maturation and block osteoblast differentiation of growth plate chondrocytes: maintains chondrocyte columnar proliferation, delays prehypertrophy and then prevents osteoblastic differentiation of chondrocytes by lowering beta-catenin (CTNNB1) signaling and RUNX2 expression (By similarity).
Also required for chondrocyte hypertrophy, both indirectly, by keeping the lineage fate of chondrocytes, and directly, by remaining present in upper hypertrophic cells and transactivating COL10A1 along with MEF2C (By similarity).
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Related Signaling Pathways
cAMP Signaling Pathway
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SOX9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3882z | Mouse Anti-SOX9 Recombinant Antibody (clone 15C2) | WB, ICC, IF, ELISA | Mouse IgG1 |
| ZG-0104C | Mouse Anti-SOX9 Recombinant Antibody (clone ABT-SOX9) | IHC-P, IF | Mouse IgG |
| VS3-CJ981 | Mouse Anti-SOX9 Recombinant Antibody (VS3-CJ981) | WB, IHC | Mouse IgG2b |
| VS3-CJ982 | Rabbit Anti-SOX9 Recombinant Antibody (VS3-CJ982) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-XY1457 | Mouse Anti-SOX9 Recombinant Antibody (clone 1B11) | ELISA, WB, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3378 | Hi-Affi™ Recombinant Rabbit Anti-SOX9 Monoclonal Antibody (DS3378AB) | WB, ICC, IF, IP | IgG |
| MOR-0106-FY | Rabbit Anti-SOX9 Recombinant Antibody (clone AFY0077) | ICC, IHC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1045 | Mouse Anti-SOX9 Recombinant Antibody (clone 334CT39.1.4) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1099 | CytoStream™ Rabbit Anti-SOX9 Recombinant Antibody (VS13-YC1099) | WB, ICC, IF, IHC-P, IP, FC, ChIP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6830 | Anti-SOX9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6831 | Anti-Mouse SOX9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6832 | Anti-Rat SOX9 Immunohistochemistry Kit | IHC |
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