CytoStream™ Rabbit Anti-SOX9 Recombinant Antibody (VS13-YC1099)
CAT#: VS13-YC1099
The CytoStream™ Rabbit Anti-SOX9 Recombinant Antibody is specific for human and mouse SOX9. SOX9 encodes a protein recognizing CCTTGAG sequences, essential for chondrocyte differentiation and AMH gene regulation; deficiencies cause campomelic dysplasia. The anti-SOX9 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22456/if_selected.jpg
Normal Tissue
Figure 2 Salivary gland.
High expression in salivary gland
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_B_1_1_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity: 75%-25% Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68240/159895_A_5_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (AN3-CA, Hep G2, OE19, U-251 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125398-SOX9
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Specifications
- Immunogen
- Recombinant human SOX9, aa 140-340.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse SOX9
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P, IP, FC, ChIP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Chromatin Immunoprecipitation (ChIP): 4 µg per 30 µg of chromatin
Target
- Alternative Names
- SRY-Box 9; Campomelic Dysplasia, Autosomal Sex-Reversal; SRY (Sex-Determining Region Y)-Box 9 Protein; SRY (Sex Determining Region Y)-Box 9; SRY (Sex Determining Region Y)-Box9; SRY-Related HMG-Box, Gene 9; Transcription Factor SOX-9;
- Gene ID
- 6662
- UniProt ID
- P48436
- Long Name
- SRY-Box 9
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_000337.1
- Function
- Transcription factor that plays a key role in chondrocytes differentiation and skeletal development (PubMed:24038782).
Specifically binds the 5'-ACAAAG-3' DNA motif present in enhancers and super-enhancers and promotes expression of genes important for chondrogenesis, including cartilage matrix protein-coding genes COL2A1, COL4A2, COL9A1, COL11A2 and ACAN, SOX5 and SOX6 (PubMed:8640233).
Also binds to some promoter regions (By similarity).
Plays a central role in successive steps of chondrocyte differentiation (By similarity).
Absolutely required for precartilaginous condensation, the first step in chondrogenesis during which skeletal progenitors differentiate into prechondrocytes (By similarity).
Together with SOX5 and SOX6, required for overt chondrogenesis when condensed prechondrocytes differentiate into early stage chondrocytes, the second step in chondrogenesis (By similarity).
Later, required to direct hypertrophic maturation and block osteoblast differentiation of growth plate chondrocytes: maintains chondrocyte columnar proliferation, delays prehypertrophy and then prevents osteoblastic differentiation of chondrocytes by lowering beta-catenin (CTNNB1) signaling and RUNX2 expression (By similarity).
Also required for chondrocyte hypertrophy, both indirectly, by keeping the lineage fate of chondrocytes, and directly, by remaining present in upper hypertrophic cells and transactivating COL10A1 along with MEF2C (By similarity).
Low lipid levels are the main nutritional determinant for chondrogenic commitment of skeletal progenitor cells: when lipids levels are low, FOXO (FOXO1 and FOXO3) transcription factors promote expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity).
Mechanistically, helps, but is not required, to remove epigenetic signatures of transcriptional repression and deposit active promoter and enhancer marks at chondrocyte-specific genes (By similarity).
Acts in cooperation with the Hedgehog pathway-dependent GLI (GLI1 and GLI3) transcription factors (By similarity).
In addition to cartilage development, also acts as a regulator of proliferation and differentiation in epithelial stem/progenitor cells: involved in the lung epithelium during branching morphogenesis, by balancing proliferation and differentiation and regulating the extracellular matrix (By similarity).
Controls epithelial branching during kidney development (By similarity).
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Related Signaling Pathways
cAMP Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SOX9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3378 | Hi-Affi™ Recombinant Rabbit Anti-SOX9 Monoclonal Antibody (DS3378AB) | WB, ICC, IF, IP | IgG |
| MOR-0106-FY | Rabbit Anti-SOX9 Recombinant Antibody (clone AFY0077) | ICC, IHC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1427-CN | Recombinant Rabbit Anti-SOX9 Monoclonal Antibody (CBACN-524) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0104C | Mouse Anti-SOX9 Recombinant Antibody (clone ABT-SOX9) | IHC-P, IF | Mouse IgG |
| VS3-CJ981 | Mouse Anti-SOX9 Recombinant Antibody (VS3-CJ981) | WB, IHC | Mouse IgG2b |
| VS3-CJ982 | Rabbit Anti-SOX9 Recombinant Antibody (VS3-CJ982) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-XY1457 | Mouse Anti-SOX9 Recombinant Antibody (clone 1B11) | ELISA, WB, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1045 | Mouse Anti-SOX9 Recombinant Antibody (clone 334CT39.1.4) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6830 | Anti-SOX9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6831 | Anti-Mouse SOX9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6832 | Anti-Rat SOX9 Immunohistochemistry Kit | IHC |
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