CytoStream™ Rabbit Anti-SOX9 Recombinant Antibody (VS13-YC1099) (CAT#: VS13-YC1099)

• Immunogen
• Recombinant human SOX9, aa 140-340.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human and Mouse SOX9

• Species Reactivity
• Human, Mouse

• Applications
• WB, ICC, IF, IHC-P, IP, FC, ChIP

• Conjugate
• NONE

• Clonality
• Monoclonal

• Purification
• Protein A purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.

• Preservative
• 0.05% Sodium azide

• Storage
• Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipping with ice pack.

• Application Notes
• Western blot (WB): 1/500
  Immunocytochemistry (ICC): 1/50-1/200
  Immunofluorescence (IF): 1/50-1/200
  Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
  Flow cytometry (FC): 1/50-1/100
  Chromatin Immunoprecipitation (ChIP): 4 µg per 30 µg of chromatin

• Alternative Names
• SRY-Box 9; Campomelic Dysplasia, Autosomal Sex-Reversal; SRY (Sex-Determining Region Y)-Box 9 Protein; SRY (Sex Determining Region Y)-Box 9; SRY (Sex Determining Region Y)-Box9; SRY-Related HMG-Box, Gene 9; Transcription Factor SOX-9;

• Gene ID
• 6662

• UniProt ID
• P48436

• Long Name
• SRY-Box 9

• Cellular Localization
• Nucleus

• Protein Refseq
• NP_000337.1

• Function
• Transcription factor that plays a key role in chondrocytes differentiation and skeletal development (PubMed:24038782).
  Specifically binds the 5'-ACAAAG-3' DNA motif present in enhancers and super-enhancers and promotes expression of genes important for chondrogenesis, including cartilage matrix protein-coding genes COL2A1, COL4A2, COL9A1, COL11A2 and ACAN, SOX5 and SOX6 (PubMed:8640233).
  Also binds to some promoter regions (By similarity).
  Plays a central role in successive steps of chondrocyte differentiation (By similarity).
  Absolutely required for precartilaginous condensation, the first step in chondrogenesis during which skeletal progenitors differentiate into prechondrocytes (By similarity).
  Together with SOX5 and SOX6, required for overt chondrogenesis when condensed prechondrocytes differentiate into early stage chondrocytes, the second step in chondrogenesis (By similarity).
  Later, required to direct hypertrophic maturation and block osteoblast differentiation of growth plate chondrocytes: maintains chondrocyte columnar proliferation, delays prehypertrophy and then prevents osteoblastic differentiation of chondrocytes by lowering beta-catenin (CTNNB1) signaling and RUNX2 expression (By similarity).
  Also required for chondrocyte hypertrophy, both indirectly, by keeping the lineage fate of chondrocytes, and directly, by remaining present in upper hypertrophic cells and transactivating COL10A1 along with MEF2C (By similarity).
  Low lipid levels are the main nutritional determinant for chondrogenic commitment of skeletal progenitor cells: when lipids levels are low, FOXO (FOXO1 and FOXO3) transcription factors promote expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity).
  Mechanistically, helps, but is not required, to remove epigenetic signatures of transcriptional repression and deposit active promoter and enhancer marks at chondrocyte-specific genes (By similarity).
  Acts in cooperation with the Hedgehog pathway-dependent GLI (GLI1 and GLI3) transcription factors (By similarity).
  In addition to cartilage development, also acts as a regulator of proliferation and differentiation in epithelial stem/progenitor cells: involved in the lung epithelium during branching morphogenesis, by balancing proliferation and differentiation and regulating the extracellular matrix (By similarity).
  Controls epithelial branching during kidney development (By similarity).

Secondary Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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