Rabbit Anti-SPARC Recombinant Antibody (VS13-YC1102) (CAT#: VS13-YC1102)
The Rabbit Anti-SPARC Recombinant Antibody is specific for human SPARC. SPARC encodes a cysteine-rich matrix-associated protein for collagen calcification in bone and ECM synthesis, linked to tumor suppression and metastasis. The anti-SPARC antibody can be used for Immunohistochemistry (1/100-1/200).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2989/1768_C6_25_cr594bb5ad76670_selected.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2989/1768_C6_25_cr594bb5ad76670_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_B_9_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_B_9_5.jpg
Figure 3 Colon
(Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_7_3.jpg
(Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_7_3.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_9_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_9_5.jpg
Figure 5 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_5_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_5_6.jpg
Figure 6 Placenta
(Cytotrophoblasts Staining: High Intensity: Strong Quantity: 75%-25% Decidual cells Staining: High Intensity: Strong Quantity:>75% Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - cell body Staining: Medium Intensity: Moderate Quantity: 75%-25% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_3_7.jpg
(Cytotrophoblasts Staining: High Intensity: Strong Quantity: 75%-25% Decidual cells Staining: High Intensity: Strong Quantity:>75% Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - cell body Staining: Medium Intensity: Moderate Quantity: 75%-25% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_3_7.jpg
Figure 7 RNA cell line category: Cell line enhanced (ASC diff, BJ, fHDF/TERT166, WM-115)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113140-SPARC
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113140-SPARC
Specifications
- Immunogen
- Recombinant human SPARC.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SPARC
- Species Reactivity
- Human
- Applications
- IHC-P
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.7) containing 50% Glycerol and 0.5% BSA.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Immunohistochemistry (IHC): 1/100-1/200
Target
- Alternative Names
- Secreted Protein Acidic And Cysteine Rich; Secreted Protein Acidic And Rich In Cysteine; Basement-Membrane Protein 40; Osteonectin; BM-40; ON; Secreted Protein, Acidic, Cysteine-Rich (Osteonectin); Cysteine-Rich Protein; OI17;
- Gene ID
- 6678
- UniProt ID
- P09486
- Long Name
- Secreted Protein Acidic And Cysteine Rich
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3572z | Mouse Anti-SPARC Recombinant Antibody (clone 41H5) | WB, IP, IF, IHC | Mouse IgG1 |
| HPAB-0267-FY | Human Anti-SPARC Recombinant Antibody (clone Imml 2) | WB, ELISA | Humanized IgG1, κ |
| HPAB-0303-FY | Human Anti-SPARC Recombinant Antibody (clone Fab6) | ELISA | Humanized IgG |
| HPAB-0304-FY | Human Anti-SPARC Recombinant Antibody (clone Fab16) | ELISA | Humanized IgG |
| VS3-QX1047 | Mouse Anti-SPARC Recombinant Antibody (clone 664CT7.3.1) | WB | Mouse IgG1 |
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