Rabbit Anti-SPARC Recombinant Antibody (VS13-YC1102) (CAT#: VS13-YC1102)
The Rabbit Anti-SPARC Recombinant Antibody is specific for human SPARC. SPARC encodes a cysteine-rich matrix-associated protein for collagen calcification in bone and ECM synthesis, linked to tumor suppression and metastasis. The anti-SPARC antibody can be used for Immunohistochemistry (1/100-1/200).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2989/1768_C6_25_cr594bb5ad76670_selected.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2989/1768_C6_25_cr594bb5ad76670_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_B_9_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_B_9_5.jpg
Figure 3 Colon
(Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_7_3.jpg
(Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_7_3.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_9_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_9_5.jpg
Figure 5 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_5_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_5_6.jpg
Figure 6 Placenta
(Cytotrophoblasts Staining: High Intensity: Strong Quantity: 75%-25% Decidual cells Staining: High Intensity: Strong Quantity:>75% Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - cell body Staining: Medium Intensity: Moderate Quantity: 75%-25% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_3_7.jpg
(Cytotrophoblasts Staining: High Intensity: Strong Quantity: 75%-25% Decidual cells Staining: High Intensity: Strong Quantity:>75% Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Hofbauer cells Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - cell body Staining: Medium Intensity: Moderate Quantity: 75%-25% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2306/6036_A_3_7.jpg
Figure 7 RNA cell line category: Cell line enhanced (ASC diff, BJ, fHDF/TERT166, WM-115)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113140-SPARC
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113140-SPARC
Specifications
- Immunogen
- Recombinant human SPARC.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SPARC
- Species Reactivity
- Human
- Applications
- IHC-P
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.7) containing 50% Glycerol and 0.5% BSA.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Immunohistochemistry (IHC): 1/100-1/200
Target
- Alternative Names
- Secreted Protein Acidic And Cysteine Rich; Secreted Protein Acidic And Rich In Cysteine; Basement-Membrane Protein 40; Osteonectin; BM-40; ON; Secreted Protein, Acidic, Cysteine-Rich (Osteonectin); Cysteine-Rich Protein; OI17;
- Gene ID
- 6678
- UniProt ID
- P09486
- Long Name
- Secreted Protein Acidic And Cysteine Rich
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3572z | Mouse Anti-SPARC Recombinant Antibody (clone 41H5) | WB, IP, IF, IHC | Mouse IgG1 |
| HPAB-0267-FY | Human Anti-SPARC Recombinant Antibody (clone Imml 2) | WB, ELISA | Humanized IgG1, κ |
| HPAB-0303-FY | Human Anti-SPARC Recombinant Antibody (clone Fab6) | ELISA | Humanized IgG |
| HPAB-0304-FY | Human Anti-SPARC Recombinant Antibody (clone Fab16) | ELISA | Humanized IgG |
| VS3-QX1047 | Mouse Anti-SPARC Recombinant Antibody (clone 664CT7.3.1) | WB | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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