Rabbit Anti-SUMO1 Recombinant Antibody (clone 5G3)
CAT#: ZG-0587U
Rabbit Anti-SUMO1 Antibody (clone 5G3) displayed high specificity against the SUMO1 protein of Human.
Tested Data
IF
Figure 1 Rabbit Anti-SUMO1 Antibody (ZG-0587U) in IF
IHC image of ZG-0587U diluted at 1:92.5 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
FC
Figure 2 Rabbit Anti-SUMO1 Antibody (ZG-0587U) in FC
IHC image of ZG-0587U diluted at 1:92.5 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
WB
Figure 3 Rabbit Anti-SUMO1 Antibody (ZG-0587U) in WB
Immunoprecipitating SUMO1 in 293T whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0587U in 293T whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0587U (3μg) + 293T whole cell lysate (500μg)
Lane 3: 293T whole cell lysate (20μg)
FC
Figure 4 Rabbit Anti-SUMO1 Antibody (ZG-0587U) in FC
Overlay histogram showing Hela cells stained with ZG-0587U (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of>10, 000 events was performed.
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Specifications
- Immunogen
- A synthesized peptide derived from human SUMO1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SUMO1
- Species Reactivity
- Human
- Clone
- 5G3
- Applications
- ELISA, IHC, FC, IP
- Conjugate
- Unconjugated
- Related Disease
- Orofacial Cleft 10 and Cleft Lip.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Flow Cytometry, Immunoprecipitation. Specific conditions should be optimized by the end user.
IHC:1:50-1:200, IP:1:200-1:1000
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "SUMO1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0267MC | Recombinant Anti-SUMO-1 Antibody | WB, IP, IF, IHC(P), ELISA | |
| MOB-0413ZL | Goat anti-Human SUMO-1 Antibody | WB, IP, IF, ELISA | |
| MRO-1452-CN | Recombinant Rabbit Anti-SUMO1 Monoclonal Antibody (CBACN-538) | WB, IF, IHC, IP, FC, ChIP | Rabbit IgG |
| VS3-CJ4 | Mouse Anti-SUMO1 Recombinant Antibody (VS3-CJ4) | WB, ELISA | Mouse IgG |
| VS3-FY1412 | Recombinant Rabbit Anti-SUMO1 Antibody (clone R04-9G9) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3460 | Hi-Affi™ Recombinant Rabbit Anti-SUMO1 Monoclonal Antibody (DS3460AB) | WB, IHC-P, ICC, IF, FC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1166 | AbPlus™ Anti-SUMO1 Magnetic Beads (VS-0724-YC1166) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY496 | Rabbit Anti-NHP SUMO1 Recombinant Antibody (VS-1024-XY496) | WB, IHC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1130 | CytoStream™ Rabbit Anti-SUMO1 Recombinant Antibody (VS13-YC1130) | WB, ICC, IF, IHC-P, IP, FC, ChIP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2228 | Anti-SUMO1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7021 | Anti-Mouse SUMO1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7020 | Anti-Human SUMO1 Immunohistochemistry Kit | IHC |
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