Rabbit Anti-SYNE3 Recombinant Antibody (clone R05-1B8)
CAT#: VS3-FY1798
This anti-Nesprin3 antibody detected positive in western blot analysis of nesprin3 in Jurkat, Hela lysates. The recombinant monoclonal antibody has been shown to be effective to target human SYNE3. Western blot demonstrated that R05-1B8 recognized a target protein with a molecular weight of 112 kDa.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nuclear membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/77140/1609_C3_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows distinct nuclear membranous and cytoplasmic positivity in cells in seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55227/127589_A_6_6_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55227/127589_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55227/127589_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55227/127589_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55227/127589_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, Karpas-707, REH)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000176438-SYNE3/subcellular
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WB
Figure 1 Anti-SYNE3 Antibody (VS3-FY1798) in WB.
The Nesprin3 expression in Jurkat, Hela lysates were detected using the anti-Nesprin3 by western blot assay.
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Specifications
- Immunogen
- Recombinant human Nesprin3 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SYNE3
- Species Reactivity
- Human
- Clone
- R05-1B8
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 112 kDa, while the observed molecular weight stands at 112 kDa.
- Related Disease
- Spermatogenic Failure 16 and Male Infertility Due To Acephalic Spermatozoa
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- KASH3; NET53; Nesp3; C14orf49; C14orf139; LINC00341; NCRNA00341
- Gene ID
- 161176
- UniProt ID
- Q6ZMZ3
- Sequence Similarities
- Belongs to the nesprin family.
- Cellular Localization
- Endoplasmic reticulum, Membrane, Nucleus
- Post Translation Modifications
- The disulfid bond with SUN1 or SUN2 is required for stability of the respective LINC complex under tensile forces. (Q6ZMZ3-SYNE3_HUMAN)
Modification sites at PhosphoSitePlus (Q6ZMZ3)
Modification sites at neXtProt (NX_Q6ZMZ3)
Glycosylation from GlyGen (Q6ZMZ3) 2 sites, 2 O-linked glycans (2 sites)
- Protein Refseq
- NP_001350621.1; NP_001371210.1; NP_001371211.1; NP_001371212.1; NP_001371213.1
- Function
- As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Syne3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-1068F | Mouse Anti-Syne3 Recombinant Antibody (clone Nsp-3) | IHC-Fr | Mouse IgG |
| VS1-YJ143 | Rabbit Anti-Syne3 Antibody (VS-0722-YJ143) | IB, IF, WB, IP | Rabbit IgG |
| VS13-YC1133 | Rabbit Anti-SYNE3 Recombinant Antibody (VS13-YC1133) | WB | Rabbit IgG |
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