Rabbit Anti-Syne3 Antibody (VS-0722-YJ143)
CAT#: VS1-YJ143
This product is a rabbit antibody that recognizes Syne3.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nuclear membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/77140/1609_C3_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows distinct nuclear membranous and cytoplasmic positivity in cells in seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55227/127589_A_6_6_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55227/127589_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55227/127589_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55227/127589_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55227/127589_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, Karpas-707, REH)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000176438-SYNE3/subcellular
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Specifications
- Immunogen
- GST-Nesprin 3 peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse Syne3
- Species Reactivity
- Mouse
- Applications
- IB, IF, WB, IP
Product Property
- Concentration
- See the COA
- Buffer
- See the COA
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Immunoblotting, Immunofluorescence, Western Blot, Immunoprecipitation.
Target
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Customer Review
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Excellent in Western Blots
I used the Anti-SYNE3 Antibody (VS-0722-YJ143) for Western blot analysis, and it worked wonderfully. The antibody detected SYNE3 in human cell lysates with clear, strong bands and minimal background noise. The results were consistent and reproducible across multiple experiments. The protocol was easy to follow, and I appreciated the detailed datasheet provided. This antibody has greatly enhanced the quality of my research.
Great for Immunofluorescence
This antibody performed exceptionally well in immunofluorescence studies. I was able to visualize the localization of SYNE3 in mouse cells with bright and specific staining. The antibody showed minimal non-specific binding, which made the images clear and easy to interpret. It's been a valuable tool for studying cellular structures in my lab.
Good Value
Considering its excellent performance, this antibody is reasonably priced. It offers great value for the quality it provides. Budget constraints are always a concern in research, and finding reliable reagents like this one helps a lot. I will definitely purchase it again for future projects.
Q&As
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What is the main target of the Anti-SYNE3 Antibody (VS-0722-YJ143)?
A: The main target of this antibody is the Synaptic Nuclear Envelope Protein 3 (SYNE3), also known as Nesprin-3. SYNE3 is a protein that plays a crucial role in linking the nuclear envelope to the cytoskeleton in cells. This connection is important for maintaining the structure and integrity of the nucleus and for various cellular functions.
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Does this antibody work with mouse or rat samples?
A: Yes, the Anti-SYNE3 Antibody (VS-0722-YJ143) is known to cross-react with SYNE3 from mouse and rat. This makes it useful for studies using rodent models to explore SYNE3's function and role in different biological processes.
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Can this antibody be used in immunoprecipitation experiments?
A: Yes, the Anti-SYNE3 Antibody (VS-0722-YJ143) is suitable for immunoprecipitation. It can help isolate SYNE3 protein from cell lysates, enabling the study of protein interactions and functions in various cellular pathways.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Syne3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-1068F | Mouse Anti-Syne3 Recombinant Antibody (clone Nsp-3) | IHC-Fr | Mouse IgG |
| VS3-FY1798 | Rabbit Anti-SYNE3 Recombinant Antibody (clone R05-1B8) | WB | Rabbit IgG |
| VS13-YC1133 | Rabbit Anti-SYNE3 Recombinant Antibody (VS13-YC1133) | WB | Rabbit IgG |
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