Mouse Anti-TERT Recombinant Antibody (clone 3H2G9)
CAT#: VS7-0425-WR963
This product is a mouse IgG1 antibody that binds to human TERT. The antibody 3H2G9 recognizes specific amino acid domain of human TERT (AA: 1029-1132). It can be used for IHC and FC assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line LHCN-M2
Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm & nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54641/1782_B1_17_cr596b5f3cb51d0_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human liver
Immunohistochemical staining of human liver shows no positivity in hepatocytes and bile duct cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54641/142147_A_9_4_selected.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54641/142147_A_7_5.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced.
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164362-TERT/subcellular
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Specifications
- Immunogen
- Recombinant human TERT (AA: 1029-1132) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human TERT
- Species Reactivity
- Human
- Clone
- 3H2G9
- Molecule Class
- Enzyme
- Applications
- IHC, FC
- Conjugate
- Unconjugated
- Related Disease
- Aplastic anemia (AA); Dyskeratosis congenita, autosomal dominant, 2 (DKCA2); Pulmonary fibrosis, and/or bone marrow failure syndrome, telomere-related, 1 (PFBMFT1); Dyskeratosis congenita, autosomal recessive, 4 (DKCB4); Melanoma, cutaneous malignant 9 (CMM9)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- TERT; telomerase reverse transcriptase; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1; telomerase catalytic subunit; telomerase-associated protein 2
- Gene ID
- 7015
- UniProt ID
- O14746
- Long Name
- Telomerase reverse transcriptase
- Cellular Localization
- Chromosome; Cytoplasm; Nucleus; Telomere
- Post Translation Modifications
- Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001180305.1
- Function
- DNA-binding; Nucleotidyltransferase; Ribonucleoprotein; RNA-directed DNA polymerase; Transferase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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