Mouse Anti-TERT Recombinant Antibody (clone 3H2G9) (CAT#: VS7-0425-WR963)
This product is a mouse IgG1 antibody that binds to human TERT. The antibody 3H2G9 recognizes specific amino acid domain of human TERT (AA: 1029-1132). It can be used for IHC and FC assays.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line LHCN-M2
(Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm & nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54641/1782_B1_17_cr596b5f3cb51d0_selected.jpg
(Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm & nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54641/1782_B1_17_cr596b5f3cb51d0_selected.jpg
Figure 2 IHC staining of human liver
(Immunohistochemical staining of human liver shows no positivity in hepatocytes and bile duct cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54641/142147_A_9_4_selected.jpg
(Immunohistochemical staining of human liver shows no positivity in hepatocytes and bile duct cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54641/142147_A_9_4_selected.jpg
Figure 3 Kidney
(Cells in tubules
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54641/142147_A_7_5.jpg
(Cells in tubules
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54641/142147_A_7_5.jpg
Figure 4 RNA cell line category: Cell line enhanced.
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164362-TERT/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164362-TERT/subcellular
Specifications
- Immunogen
- Recombinant human TERT (AA: 1029-1132) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human TERT
- Species Reactivity
- Human
- Clone
- 3H2G9
- Molecule Class
- Enzyme
- Applications
- IHC, FC
- Conjugate
- Unconjugated
- Related Disease
- Aplastic anemia (AA); Dyskeratosis congenita, autosomal dominant, 2 (DKCA2); Pulmonary fibrosis, and/or bone marrow failure syndrome, telomere-related, 1 (PFBMFT1); Dyskeratosis congenita, autosomal recessive, 4 (DKCB4); Melanoma, cutaneous malignant 9 (CMM9)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- TERT; telomerase reverse transcriptase; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1; telomerase catalytic subunit; telomerase-associated protein 2
- Gene ID
- 7015
- UniProt ID
- O14746
- Long Name
- Telomerase reverse transcriptase
- Cellular Localization
- Chromosome; Cytoplasm; Nucleus; Telomere
- Post Translation Modifications
- Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001180305.1
- Function
- DNA-binding; Nucleotidyltransferase; Ribonucleoprotein; RNA-directed DNA polymerase; Transferase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-962-F(E) | Recombinant Anti-human TERT Antibody Fab Fragment | ELISA, RIA, FuncS | Fab |
| MHH-962-F(E) | Recombinant Human Anti-human TERT Antibody Fab Fragment | WB, IHC, FuncS | Fab |
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