Human Anti-Testosterone Recombinant Antibody (clone Test) (CAT#: FAMAB-0027JF)
Recombinant monoclonal antibody can specially bind to human testosterone. This anti-testosterone antibody (Test) is useful as a widely used marker to detect osteoporosis.
Specific Inquiry
IHC
Figure 1 Significant myocardial hypertrophy of rats that received testosterone.
No apoptosis detected in the control rats. ×125.
Papamitsou, T., Barlagiannis, D., Papaliagkas, V., Kotanidou, E., & Dermentzopoulou-Theodoridou, M. (2011). Testosterone-induced hypertrophy, fibrosis and apoptosis of cardiac cells–an ultrastructural and immunohistochemical study. Medical science monitor: international medical journal of experimental and clinical research, 17(9), BR266.
ELISA
Figure 2 Standard curve for plasma testosterone ELISA.
Parallelism is shown for different volumes of pathological samples.
Elder, P. A., & Lewis, J. G. (1985). An enzyme-linked immunosorbent assay (ELISA) for plasma testosterone. Journal of steroid biochemistry, 22(5), 635-638.
FC
Figure 3 Flow cytometry of CD4/ and CD8/ T cells labeled with testosterone-BSA-FITC.
Intact T cells were simultaneously labeled with testosterone-BSA-FITC and CD4-phycoerythrin (PE) or CD8-PE.
Benten, W. P. M., Lieberherr, M., Giese, G., Wrehlke, C., Stamm, O., Sekeris, C. E., ... & Wunderlich, F. (1999). Functional testosterone receptors in plasma membranes of T cells. The FASEB journal, 13(1), 123-133.
WB
Figure 4 Detection of AR in T cells by Western blotting.
Western blot of LNCaP cells and T cells performed with the anti-AR antibody AR.
Benten, W. P. M., Lieberherr, M., Giese, G., Wrehlke, C., Stamm, O., Sekeris, C. E., ... & Wunderlich, F. (1999). Functional testosterone receptors in plasma membranes of T cells. The FASEB journal, 13(1), 123-133.
Specifications
- Host Species
- Human
- Derivation
- Human phage display scFv library
- Type
- Human IgG
- Specificity
- Human testosterone
- Species Reactivity
- Human
- Clone
- Test
- Applications
- WB, ELISA, IHC, FC
- Related Disease
- Osteoporosis
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The Testosterone antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Flow Cytometry.
Target
- Alternative Names
- Testosterone
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-963-S(P) | Recombinant Anti-Testosterone Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| MHH-963-S(P) | Recombinant Human Anti-Testosterone Antibody scFv Fragment | WB, IHC, FuncS | scFv |
| FAMAB-0283-YC-S(P) | Human Anti-Testosterone Recombinant Antibody (clone TC-5); scFv Fragment | ELISA | Human scFv |
| FAMAB-0284-YC-S(P) | Human Anti-Testosterone Recombinant Antibody (clone TC-20); scFv Fragment | ELISA | Human scFv |
| FAMAB-0027JF-S(P) | Human Anti-Testosterone Recombinant Antibody (clone Test); scFv Fragment | WB, ELISA, FuncS | Human scFv |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-100 | Recombinant Anti-human Testosterone VHH Single Domain Antibody | WB, IHC, FC, FuncS | Llama VHH |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-963-F(E) | Recombinant Human Anti-Testosterone Antibody Fab Fragment | ELISA, WB, IF, FuncS | Fab |
| PFBC-120 | Mouse Anti-Testosterone Recombinant Antibody (clone 3-C(4)F(5)); Fab Fragment | WB, FuncS | Mouse Fab |
| PFBZ-120 | Mouse Anti-Testosterone Recombinant Antibody (clone 3-C4F5); Fab Fragment | Block | Mouse Fab |
| PFBC-121 | Mouse Anti-Testosterone Recombinant Antibody (clone 3-C4F5 mutant (77)); Fab Fragment | WB, ELISA | Mouse Fab |
| FAMAB-0283-YC-F(E) | Human Anti-Testosterone Recombinant Antibody (clone TC-5); Fab Fragment | ELISA | Human Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-120 | Mouse Anti-Testosterone Recombinant Antibody (clone 3-C(4)F(5)) | WB, FuncS | Mouse IgG |
| PABZ-120 | Mouse Anti-Testosterone Recombinant Antibody (clone 3-C4F5) | Block | Mouse IgG |
| PABC-121 | Mouse Anti-Testosterone Recombinant Antibody (clone 3-C4F5 mutant (77)) | WB, ELISA | Mouse IgG |
| FAMAB-0301-CN | Human Anti-Testosterone Recombinant Antibody (clone TM-7) | ELISA | Human IgG |
| FAMAB-0302-CN | Human Anti-Testosterone Recombinant Antibody (clone TM-4) | ELISA | Human IgG |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-176 | Recombinant Mouse Anti-Testosterone Antibody (77Fab) | Neut, FuncS | IgG |
| PABX-176-S (P) | Recombinant Mouse Anti-Testosterone Antibody scFv Fragment (77Fab) | Neut, FuncS | scFv |
| PABX-176-F (E) | Recombinant Mouse Anti-Testosterone Antibody Fab Fragment (77Fab) | Neut, FuncS | Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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