Rabbit Anti-TFF3 Recombinant Antibody (clone R06-6J1) (CAT#: VS3-XY3524)
This rabbit recombinant antibody specifically recognizes TFF3 (Trefoil factor 3), which involves in the maintenance and repair of the intestinal mucosa. The antibody specifically reacts with human TFF3. It has been tested using Western Blot, Immunohistochemistry-Paraffin and Immunoprecipitation. The recommended dilutions of Western blot, Immunohistochemistry-Paraffin and Immunoprecipitation is 1/500-1/1000, 1/50-1/100 and 1/20, respectively.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35464/1134_F11_5_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35464/1134_F11_5_selected.jpg
Figure 2 Rectum
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35464/165692_A_4_3_val_selected.jpg
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35464/165692_A_4_3_val_selected.jpg
Figure 3 Small intestine
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20681/ihc_selected.jpg
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20681/ihc_selected.jpg
Figure 4 Colon
(Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35464/165692_A_7_3.jpg
(Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35464/165692_A_7_3.jpg
Figure 5 RNA cell line category: Cell line enhanced (MCF7, OE19, T-47d, TIME)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160180-TFF3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160180-TFF3
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TFF3
- Species Reactivity
- Human
- Clone
- R06-6J1
- Applications
- WB, IHC-P, IP
- Related Disease
- Intellectual Developmental Disorder
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IP: 1/20
Target
- Alternative Names
- ITF; P1B; TFI
- Gene ID
- 7033
- UniProt ID
- Q07654
- Long Name
- Trefoil factor 3
- Cellular Localization
- Cytoplasm, Cytosol, Nucleus
- Post Translation Modifications
- Sumoylated; does not affect dimerization with MITF. (P19532-TFE3_HUMAN)
Phosphorylation ar Ser-47 and Ser-321 by MTOR via non-canonical mTORC1 pathway regulates its stability and subcellular location, respectively.
- Protein Refseq
- XP_054183633.1
- Function
- Transcription factor that acts as a master regulator of lysosomal biogenesis and immune response (PubMed:2338243, 24448649, 29146937, 30733432, 31672913, 37079666).
Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF (PubMed:24448649).
Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFE3 phosphorylation by MTOR promotes its inactivation.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0834MZ | Recombinant Mouse Anti-Human TFF3 Antibody | IHC-P | Mouse antibody |
| HPAB-0133-CN | Human Anti-TFF3 Recombinant Antibody (clone 1) | ELISA | Human IgG |
| ZG-0180U | Mouse Anti-TFF3 Recombinant Antibody (clone 1D3G1) | ELISA, IHC, IF | Mouse IgG2a |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3545 | Rabbit Anti-TFF3 Recombinant Antibody (clone DS3545AB) | ICC, IHC-P, WB | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0133-CN-S(P) | Human Anti-TFF3 Recombinant Antibody (clone 1); scFv Fragment | ELISA | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0133-CN-F(E) | Human Anti-TFF3 Recombinant Antibody (clone 1); Fab Fragment | ELISA | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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