Mouse Anti-TFIIB Recombinant Antibody (VS7-0425-WR4) (CAT#: VS7-0425-WR4)

• Immunogen
• Recombinant human TFIIB fragment expressed in E. Coli.

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human, Mouse, Rat, Bovine, Chicken, Dog, Pig TFIIB

• Species Reactivity
• Human, Mouse, Rat, Bovine, Chicken, Dog, Pig

• Molecule Class
• Transcription Factor

• Applications
• WB, IHC-P, IF, ICC

• Conjugate
• Unconjugated

• Related Disease
• Spinocerebellar ataxia 17; Cockayne syndrome; Xeroderma pigmentosum.

• Clonality
• Monoclonal

• Purification
• Affinity purification

• Purity
• >95% as determined by SDS-PAGE and SEC-HPLC analysis

• Format
• Liquid

• Concentration
• 1 mg/mL

• Buffer
• 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl)

• Preservative
• 0.2% sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Immunofluorescence: 1:100 - 1:500
  Immunohistochemistry: 1:500 - 1:1000
  Western Blot: 1:1000 - 1:2000

• Alternative Names
• TF2B; TFIIB; GTF2B; general transcription factor IIB; transcription factor II B

• Gene ID
• 2959

• UniProt ID
• Q00403

• Long Name
• Transcription factor II B

• Cellular Localization
• Chromosome; Nucleus

• Post Translation Modifications
• Acetylation; Phosphorylation

• Protein Refseq
• NP_001505

• Function
• General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:12931194, PubMed:1517211, PubMed:1876184, PubMed:1946368, PubMed:27193682, PubMed:3029109, PubMed:3818643, PubMed:7601352, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:9420329).; Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8413225, PubMed:8504927, PubMed:8515820, PubMed:8516311, PubMed:8516312).; Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352).; Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171).; Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:10619841, PubMed:16230532, PubMed:7675079, PubMed:9420329).; Modulates transcription start site selection (PubMed:10318856).; Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194).

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

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