Mouse Anti-TFIIB Recombinant Antibody (VS7-0425-WR4) (CAT#: VS7-0425-WR4)

This product is a recombinant mouse IgG antibody that binds human TFIIB. The antibody can be used for WB, IHC-P, IF, and ICC assays. The antibody also cross reacts with other species including mouse, rat, bovine, chicken, dog, and pig.

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Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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We require custom production
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Normal Tissue
RNA Expression

Specifications

  • Immunogen
  • Recombinant human TFIIB fragment expressed in E. Coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human, Mouse, Rat, Bovine, Chicken, Dog, Pig TFIIB
  • Species Reactivity
  • Human, Mouse, Rat, Bovine, Chicken, Dog, Pig
  • Molecule Class
  • Transcription Factor
  • Applications
  • WB, IHC-P, IF, ICC
  • Conjugate
  • Unconjugated
  • Related Disease
  • Spinocerebellar ataxia 17; Cockayne syndrome; Xeroderma pigmentosum.

Product Property

  • Clonality
  • Monoclonal
  • Purification
  • Affinity purification
  • Purity
  • >95% as determined by SDS-PAGE and SEC-HPLC analysis
  • Format
  • Liquid
  • Concentration
  • 1 mg/mL
  • Buffer
  • 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl)
  • Preservative
  • 0.2% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • Immunofluorescence: 1:100 - 1:500
    Immunohistochemistry: 1:500 - 1:1000
    Western Blot: 1:1000 - 1:2000

Target

  • Alternative Names
  • TF2B; TFIIB; GTF2B; general transcription factor IIB; transcription factor II B
  • Long Name
  • Transcription factor II B
  • Cellular Localization
  • Chromosome; Nucleus
  • Post Translation Modifications
  • Acetylation; Phosphorylation
  • Function
  • General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:12931194, PubMed:1517211, PubMed:1876184, PubMed:1946368, PubMed:27193682, PubMed:3029109, PubMed:3818643, PubMed:7601352, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:9420329).; Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8413225, PubMed:8504927, PubMed:8515820, PubMed:8516311, PubMed:8516312).; Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352).; Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171).; Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:10619841, PubMed:16230532, PubMed:7675079, PubMed:9420329).; Modulates transcription start site selection (PubMed:10318856).; Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194).

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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