Mouse Anti-TFIIB Recombinant Antibody (VS7-0425-WR4)
CAT#: VS7-0425-WR4
This product is a recombinant mouse IgG antibody that binds human TFIIB. The antibody can be used for WB, IHC-P, IF, and ICC assays. The antibody also cross reacts with other species including mouse, rat, bovine, chicken, dog, and pig.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/1153_H2_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/140313_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/140313_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/140313_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/140313_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61626/140313_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000137947-GTF2B
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Specifications
- Immunogen
- Recombinant human TFIIB fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse, Rat, Bovine, Chicken, Dog, Pig TFIIB
- Species Reactivity
- Human, Mouse, Rat, Bovine, Chicken, Dog, Pig
- Molecule Class
- Transcription Factor
- Applications
- WB, IHC-P, IF, ICC
- Conjugate
- Unconjugated
- Related Disease
- Spinocerebellar ataxia 17; Cockayne syndrome; Xeroderma pigmentosum.
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purification
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl)
- Preservative
- 0.2% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Immunofluorescence: 1:100 - 1:500
Immunohistochemistry: 1:500 - 1:1000
Western Blot: 1:1000 - 1:2000
Target
- Alternative Names
- TF2B; TFIIB; GTF2B; general transcription factor IIB; transcription factor II B
- Gene ID
- 2959
- UniProt ID
- Q00403
- Long Name
- Transcription factor II B
- Cellular Localization
- Chromosome; Nucleus
- Post Translation Modifications
- Acetylation; Phosphorylation
- Protein Refseq
- NP_001505
- Function
- General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:12931194, PubMed:1517211, PubMed:1876184, PubMed:1946368, PubMed:27193682, PubMed:3029109, PubMed:3818643, PubMed:7601352, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:9420329).; Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8413225, PubMed:8504927, PubMed:8515820, PubMed:8516311, PubMed:8516312).; Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352).; Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171).; Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:10619841, PubMed:16230532, PubMed:7675079, PubMed:9420329).; Modulates transcription start site selection (PubMed:10318856).; Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "GTF2B"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2012CT | Recombinant Mouse anti-Human GTF2B Monoclonal antibody (JJC2) | ChIP, IP, WB | |
| VS3-QX489 | Mouse Anti-GTF2B Recombinant Antibody (clone 2F6-A3) | WB | Mouse IgG2b |
| VS3-CJ27 | Mouse Anti-GTF2B Recombinant Antibody (VS3-CJ27) | WB, ELISA | Mouse IgG |
| VS4-WK206 | Mouse Anti-GTF2B Recombinant Antibody (VS4-WK206) | ELISA, WB, IHC, ICC | Mouse IgG |
| VS13-YC484 | Rabbit Anti-GTF2B Recombinant Antibody (VS13-YC484) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1539 | Rabbit Anti-GTF2B Recombinant Antibody (clone DS1539AB) | WB, IHC, IP, FC | Rabbit IgG |
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