Human Anti-TGFA Recombinant Antibody (clone LY3016859) (CAT#: PABC-538)
This product is a recombinant Human antibody that can recognize TGFA. LY3016859, a humanized monoclonal hIgG4 antibody, which binds and neutralizes only TGFα and epiregulin with high affinity.
Specific Inquiry
ELISA
Figure 1 LY3016859 inhibit Tgfa mediated cell proliferation.
Inhibition of human Tgfa by LY3016859 in a cell proliferation BrDU colorimetric ELISA assay. The data shown represent the mean ± the SEM. N=4 replicate wells per concentration. The dashed line represents basal proliferation in the absence of ligand. Ligand concentrations were 0.5 nM.
Beidler, C. B., Petrovan, R. J., Conner, E. M., Boyles, J. S., Yang, D. D., Harlan, S. M., ... & Stauber, A. (2014). Generation and activity of a humanized monoclonal antibody that selectively neutralizes the epidermal growth factor receptor ligands transforming growth factor-α and epiregulin. Journal of Pharmacology and Experimental Therapeutics, 349(2), 330-343.
WB
Figure 2 Five concentrations of recombinant soluble human Tgfa were tested for binding to LY3016859 by Western blot under non-reducing and reducing conditions.
Beidler, C. B., Petrovan, R. J., Conner, E. M., Boyles, J. S., Yang, D. D., Harlan, S. M., ... & Stauber, A. (2014). Generation and activity of a humanized monoclonal antibody that selectively neutralizes the epidermal growth factor receptor ligands transforming growth factor-α and epiregulin. Journal of Pharmacology and Experimental Therapeutics, 349(2), 330-343.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG4
- Specificity
- Human TGFA
- Species Reactivity
- Human
- Clone
- LY3016859
- Applications
- ELISA, FC, Neut, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry, Western Blot and Neutralization. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- TGFA; transforming growth factor; alpha; TFGA; protransforming growth factor alpha; TGF-alpha; anti-TGFalpha
- Gene ID
- 7039
- UniProt ID
- P01135
Related Resources
Non-small Cell Lung Cancer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone LY3016859"
See other products for "TGFA"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1199z | Mouse Anti-TGFA Recombinant Antibody (clone 12B8) | WB, IF, IP | Mouse IgG1 |
| PABZ-122 | Mouse Anti-TGFA Recombinant Antibody (clone tAb2) | WB, FuncS | Mouse IgG |
| MOB-2017CT | Recombinant Mouse anti-Human TGFA Monoclonal antibody (Q/U2) | ELISA, ICC, IHC-Fr, IHC-P | |
| HPAB-0544-CN | Mouse Anti-TGFA Recombinant Antibody (HPAB-0544-CN) | WB, ELISA, FC, IHC, IP, Neut | Mouse IgG1, κ |
| VS3-FY1445 | Recombinant Rabbit Anti-TGFA Antibody (clone R07-6J8) | WB, IHC-P, IP | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-122 | Mouse Anti-TGFA Recombinant Antibody (clone tAb2); Fab Fragment | WB, FuncS | Mouse Fab |
| HPAB-0544-CN-F(E) | Mouse Anti-TGFA Recombinant Antibody; Fab Fragment (HPAB-0544-CN-F(E)) | WB, ELISA, FC, IHC, IP, Neut | Mouse Fab |
| HPAB-0545-CN-F(E) | Mouse Anti-TGFA Recombinant Antibody; Fab Fragment (HPAB-0545-CN-F(E)) | WB, ELISA, FC, IHC, IP, Neut | Mouse Fab |
| HPAB-0546-CN-F(E) | Mouse Anti-TGFA Recombinant Antibody; Fab Fragment (HPAB-0546-CN-F(E)) | WB, ELISA, FC, IHC, IP, Neut | Mouse Fab |
| PFBC-538 | Human Anti-TGFA Recombinant Antibody (clone LY3016859); Fab Fragment | ELISA, FC, Neut | Humanized Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-122 | Mouse Anti-TGFA Recombinant Antibody (clone tAb2); scFv Fragment | WB, FuncS | Mouse scFv |
| HPAB-0544-CN-S(P) | Mouse Anti-TGFA Recombinant Antibody; scFv Fragment (HPAB-0544-CN-S(P)) | WB, ELISA, FC, IHC, IP, Neut | Mouse scFv |
| HPAB-0545-CN-S(P) | Mouse Anti-TGFA Recombinant Antibody; scFv Fragment (HPAB-0545-CN-S(P)) | WB, ELISA, FC, IHC, IP, Neut | Mouse scFv |
| HPAB-0546-CN-S(P) | Mouse Anti-TGFA Recombinant Antibody; scFv Fragment (HPAB-0546-CN-S(P)) | WB, ELISA, FC, IHC, IP, Neut | Mouse scFv |
| PSBC-538 | Human Anti-TGFA Recombinant Antibody (clone LY3016859); scFv Fragment | ELISA, FC, Neut | Humanized scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0533CL | Mouse Anti-TGFA Recombinant Antibody (TAB-0533CL) | ELISA, Inhib | Mouse IgG |
| TAB-0533CL-S(P) | Mouse Anti-TGFA Recombinant Antibody; scFv Fragment (TAB-0533CL-S(P)) | ELISA, Inhib | Mouse scFv |
| TAB-0533CL-F(E) | Mouse Anti-TGFA Recombinant Antibody; Fab Fragment (TAB-0533CL-F(E)) | ELISA, Inhib | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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