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    Human Anti-TGFA Recombinant Antibody (PABC-538) (CAT#: PABC-538)

    This product is a recombinant human antibody that can recognize TGFA. This antibody is a humanized monoclonal hIgG4 antibody, which binds and neutralizes only TGFα and epiregulin with high affinity.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-251 MG
    Figure 1 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/if_selected.jpg
    Figure 2 IHC staining of human rectum
    Figure 2 IHC staining of human rectum
    (Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/ihc_selected.jpg
    Figure 3 IF staining of human cell line U-2 OS
    Figure 3 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/476_C8_2_blue_red_green.jpg
    Normal Tissue
    Figure 4 Cerebral cortex
    Figure 4 Cerebral cortex
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glial cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Neuronal cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_B_9_5.jpg
    Figure 5 Colon
    Figure 5 Colon
    (Glandular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Peripheral nerve/ganglion
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_3.jpg
    Figure 6 Liver
    Figure 6 Liver
    (Hepatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_4.jpg
    Figure 7 Kidney
    Figure 7 Kidney
    (Cells in tubules
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_5.jpg
    Figure 8 Testis
    Figure 8 Testis
    (Cells in seminiferous ducts
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Leydig cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_4_6.jpg
    Figure 9 Lymph node
    Figure 9 Lymph node
    (Non-germinal center cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_8.jpg
    RNA Expression
    Figure 10 RNA cell line category: Cell line enhanced (A-431, CAPAN-2, HBEC3-KT, OE19, RPTEC TERT1, U-87 MG)
    Figure 10 RNA cell line category: Cell line enhanced (A-431, CAPAN-2, HBEC3-KT, OE19, RPTEC TERT1, U-87 MG)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163235-TGFA

    Specifications

    • Host Species
    • Human
    • Derivation
    • Humanized
    • Type
    • Humanized IgG4
    • Specificity
    • Human TGFA
    • Species Reactivity
    • Human
    • Applications
    • ELISA, FC, Neut, WB

    Product Property

    • Purity
    • >95% as determined by SDS-PAGE and HPLC analysis
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry, Western Blot and Neutralization. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.

    Target

    • Alternative Names
    • TGFA; transforming growth factor; alpha; TFGA; protransforming growth factor alpha; TGF-alpha; anti-TGFalpha

    Related Resources

    • Related Diseases

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "TGFA"

    Select a product category from the dropdown menu below to view related products.
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    IgG Antibody Products Fab Antibody Products ScFv Antibody Products Mouse Antibody Products Antibody Magnetic Beads Extracellular Vesicle (EV) Antibody Products
    CAT Product Name Application Type
    PABZ-122 Mouse Anti-TGFA Recombinant Antibody (clone tAb2) WB, FuncS Mouse IgG
    MOB-2017CT Recombinant Mouse anti-Human TGFA Monoclonal antibody (Q/U2) ELISA, ICC, IHC-Fr, IHC-P
    HPAB-0544-CN Mouse Anti-TGFA Recombinant Antibody (HPAB-0544-CN) WB, ELISA, FC, IHC, IP, Neut Mouse IgG1, κ
    HPAB-0545-CN Mouse Anti-TGFA Recombinant Antibody (HPAB-0545-CN) WB, ELISA, FC, IHC, IP, Neut Mouse IgG1, κ
    HPAB-0546-CN Mouse Anti-TGFA Recombinant Antibody (HPAB-0546-CN) WB, ELISA, FC, IHC, IP, Neut Mouse IgG2a, κ

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    Versatile Research Application
    We've utilized this antibody in a variety of applications, from Western blots to ELISA. Its versatility in detecting TGFA has significantly enhanced our ability to investigate the protein's role in various biological contexts, including cancer research.

    Boosts Cancer Research Potential
    Our research into TGFA's role in cancer has greatly benefited from using this antibody. It provides clarity and precision in detecting TGFA expression, offering potential insights into tumor growth and possible intervention points.

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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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