Human Anti-TGFA Recombinant Antibody (PABC-538)
CAT#: PABC-538
This product is a recombinant human antibody that can recognize TGFA. This antibody is a humanized monoclonal hIgG4 antibody, which binds and neutralizes only TGFα and epiregulin with high affinity.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/476_C8_2_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_4_6.jpg
Normal Tissue
Figure 9 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42297/94418_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (A-431, CAPAN-2, HBEC3-KT, OE19, RPTEC TERT1, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163235-TGFA
❮
❯
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG4
- Specificity
- Human TGFA
- Species Reactivity
- Human
- Applications
- ELISA, FC, Neut, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry, Western Blot and Neutralization. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for PABC-538. Click the button above to contact us or submit your feedback about this product.
Versatile Research Application
We've utilized this antibody in a variety of applications, from Western blots to ELISA. Its versatility in detecting TGFA has significantly enhanced our ability to investigate the protein's role in various biological contexts, including cancer research.
Boosts Cancer Research Potential
Our research into TGFA's role in cancer has greatly benefited from using this antibody. It provides clarity and precision in detecting TGFA expression, offering potential insights into tumor growth and possible intervention points.
Q&As
-
What is the main application of this antibody?
A: This anti-TGFA recombinant antibody is primarily used in research to study the Transforming Growth Factor Alpha (TGFA). It is suitable for applications like Western blot and enzyme-linked immunosorbent assays (ELISA) to analyze TGFA's role in cellular growth and differentiation.
-
How should the antibody be stored for optimal use?
A: For the best results, store this antibody at 4°C for short-term use. For long-term storage, it's recommended to aliquot and freeze it at -20°C or -80°C, ensuring that multiple freeze-thaw cycles are avoided to maintain its quality and effectiveness.
-
Why study TGFA with this antibody?
A: TGFA is crucial in cell signaling related to growth and development. Using this antibody helps scientists explore TGFA's involvement in processes like tissue regeneration and cancerous growth, paving the way for innovative therapeutic approaches.
View the frequently asked questions answered by Creative Biolabs Support.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Diseases
Non-small Cell Lung Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "TGFA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1199z | Mouse Anti-TGFA Recombinant Antibody (clone 12B8) | WB, IF, IP | Mouse IgG1 |
| MOB-2017CT | Recombinant Mouse anti-Human TGFA Monoclonal antibody (Q/U2) | ELISA, ICC, IHC-Fr, IHC-P | |
| HPAB-0544-CN | Mouse Anti-TGFA Recombinant Antibody (HPAB-0544-CN) | WB, ELISA, FC, IHC, IP, Neut | Mouse IgG1, κ |
| HPAB-0545-CN | Mouse Anti-TGFA Recombinant Antibody (HPAB-0545-CN) | WB, ELISA, FC, IHC, IP, Neut | Mouse IgG1, κ |
| HPAB-0546-CN | Mouse Anti-TGFA Recombinant Antibody (HPAB-0546-CN) | WB, ELISA, FC, IHC, IP, Neut | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-122 | Mouse Anti-TGFA Recombinant Antibody (clone tAb2); Fab Fragment | WB, FuncS | Mouse Fab |
| HPAB-0544-CN-F(E) | Mouse Anti-TGFA Recombinant Antibody; Fab Fragment (HPAB-0544-CN-F(E)) | WB, ELISA, FC, IHC, IP, Neut | Mouse Fab |
| HPAB-0545-CN-F(E) | Mouse Anti-TGFA Recombinant Antibody; Fab Fragment (HPAB-0545-CN-F(E)) | WB, ELISA, FC, IHC, IP, Neut | Mouse Fab |
| HPAB-0546-CN-F(E) | Mouse Anti-TGFA Recombinant Antibody; Fab Fragment (HPAB-0546-CN-F(E)) | WB, ELISA, FC, IHC, IP, Neut | Mouse Fab |
| PFBC-538 | Human Anti-TGFA Recombinant Antibody Fab Fragment (PFBC-538) | ELISA, FC, Neut | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-122 | Mouse Anti-TGFA Recombinant Antibody (clone tAb2); scFv Fragment | WB, FuncS | Mouse scFv |
| HPAB-0544-CN-S(P) | Mouse Anti-TGFA Recombinant Antibody; scFv Fragment (HPAB-0544-CN-S(P)) | WB, ELISA, FC, IHC, IP, Neut | Mouse scFv |
| HPAB-0545-CN-S(P) | Mouse Anti-TGFA Recombinant Antibody; scFv Fragment (HPAB-0545-CN-S(P)) | WB, ELISA, FC, IHC, IP, Neut | Mouse scFv |
| HPAB-0546-CN-S(P) | Mouse Anti-TGFA Recombinant Antibody; scFv Fragment (HPAB-0546-CN-S(P)) | WB, ELISA, FC, IHC, IP, Neut | Mouse scFv |
| PSBC-538 | Human Anti-TGFA Recombinant Antibody scFv Fragment (PSBC-538) | ELISA, FC, Neut | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0533CL | Mouse Anti-TGFA Recombinant Antibody (TAB-0533CL) | ELISA, Inhib | Mouse IgG |
| TAB-0533CL-S(P) | Mouse Anti-TGFA Recombinant Antibody; scFv Fragment (TAB-0533CL-S(P)) | ELISA, Inhib | Mouse scFv |
| TAB-0533CL-F(E) | Mouse Anti-TGFA Recombinant Antibody; Fab Fragment (TAB-0533CL-F(E)) | ELISA, Inhib | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY251 | AbPlus™ Anti-TGFA Magnetic Beads (VS-0424-XY251) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC553 | Recombinant Anti-TGFA Vesicular Antibody, EV Displayed (VS-0425-YC553) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7205 | Anti-TGFA Immunohistochemistry Kit | IHC | |
| VS-0525-XY7206 | Anti-Mouse TGFA Immunohistochemistry Kit | IHC | |
| VS-0525-XY7207 | Anti-Human TGFA Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, IHC, FC, IP, IF, FuncS
-3.jpg)
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
