Mouse Anti-TGFB2 Recombinant Antibody (VS3-CJ1082) (CAT#: VS3-CJ1082)

• Immunogen
• Recombinant protein of TGFB2

• Host Species
• Mouse

• Type
• Mouse IgG1, κ

• Specificity
• Human TGFB2

• Species Reactivity
• Human

• Applications
• WB, ICC, IHC

• Conjugate
• Unconjugated

• Purification
• Protein G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• PBS

• Preservative
• 0.09% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot (1:500-1:1000), Immunocytochemistry (1:10-1:50), Immunohistochemistry (1:10-1:50).

• Alternative Names
• LDS4; G-TSF; TGF-beta2

• Gene ID
• 7042

• UniProt ID
• P61812

• Sequence Similarities
• Belongs to the TGF-beta family.

• Cellular Localization
• Secreted

• Post Translation Modifications
• Transforming growth factor beta-2 proprotein:
  The precursor proprotein is cleaved in the Golgi apparatus to form Transforming growth factor beta-2 (TGF-beta-2) and Latency-associated peptide (LAP) chains, which remain non-covalently linked, rendering TGF-beta-2 inactive.

• Protein Refseq
• NP_001129071.1; NP_003229.1

• Function
• Transforming growth factor beta-2 proprotein:
  Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively.
  Latency-associated peptide:
  Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix (By similarity).
  Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 (By similarity).
  Transforming growth factor beta-2:
  Multifunctional protein that regulates various processes such as angiogenesis and heart development (PubMed:22772371, PubMed:22772368).
  Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix (By similarity).
  At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus (By similarity).
  Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (By similarity).

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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