Mouse Anti-THBS1 Recombinant Antibody (clone 5E2E2) (CAT#: VS7-0425-WR983)
This product is a mouse IgG1 antibody that binds to human THBS1. The antibody 5E2E2 recognizes specific amino acid domain of human THBS1 (AA: 750-850). It can be used for FC assay.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/59756/1599_C12_3_selected.jpg

(Immunohistochemical staining of human small intestine shows distinct membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33678/ihc_selected.jpg

(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33678/85103_B_9_5.jpg

(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33678/85103_A_8_3.jpg

(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33678/85103_A_8_5.jpg

(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33678/85103_A_6_6.jpg

(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Lymphoid tissue Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33678/85103_A_1_2.jpg

(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33678/85103_A_7_8.jpg

(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000137801-THBS1
Specifications
- Immunogen
- Recombinant human THBS1 (AA: 750-850) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human THBS1
- Species Reactivity
- Human
- Clone
- 5E2E2
- Molecule Class
- Matricellular Glycoprotein
- Applications
- FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- THBS1; THBS-1; TSP1; Thrombospondin-1p180; Thrombospondin 1; Thrombospondin-1; TSP; TSP-1
- Gene ID
- 7057
- UniProt ID
- P07996
- Long Name
- THBS1 Gene(Protein Coding) Thrombospondin 1
- Cellular Localization
- Endoplasmic reticulum; Extracellular matrix; Sarcoplasmic reticulum; Secreted
- Post Translation Modifications
- Disulfide bond; Glycoprotein
- Protein Refseq
- NP_003237.2
- Function
- Heparin-binding
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Related Resources
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "THBS1"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1840z | Mouse Anti-THBS1 Recombinant Antibody (clone 23D12) | WB, ICC, IF, IHC, IP, Neut | Mouse IgG1 |
FAMAB-0096JF | Human Anti-THBS1 Recombinant Antibody (clone TSP-A10) | WB, IHC, ICC | Human IgG |
ZG-508C | Mouse Anti-THBS1 Recombinant Antibody (clone 1.77) | ELISA | Mouse IgG1, κ |
VS3-WK35 | Mouse Anti-THBS1 Recombinant Antibody (clone E2-E2) | WB, ELISA | Mouse IgG1 |
VS3-FY1450 | Recombinant Rabbit Anti-THBS1 Antibody (clone R06-5F3) | WB, IHC-P, IP | Rabbit IgG |
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