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    Mouse Anti-THBS1 Recombinant Antibody (clone 5E2E2) (CAT#: VS7-0425-WR983)

    This product is a mouse IgG1 antibody that binds to human THBS1. The antibody 5E2E2 recognizes specific amino acid domain of human THBS1 (AA: 750-850). It can be used for FC assay.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

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    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
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    Conjugation :
    Fc Engineering :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line BJ
    Figure 1 IF staining of human cell line BJ
    (Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/59756/1599_C12_3_selected.jpg
    Normal Tissue
    Figure 2 IHC staining of human small intestine
    Figure 2 IHC staining of human small intestine
    (Immunohistochemical staining of human small intestine shows distinct membranous positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/33678/ihc_selected.jpg
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/33678/85103_B_9_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/33678/85103_A_8_3.jpg
    Figure 5 Kidney
    Figure 5 Kidney
    (Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/33678/85103_A_8_5.jpg
    Figure 6 Testis
    Figure 6 Testis
    (Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/33678/85103_A_6_6.jpg
    Figure 7 Appendix
    Figure 7 Appendix
    (Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Lymphoid tissue Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/33678/85103_A_1_2.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/33678/85103_A_7_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Cell line enhanced (fHDF/TERT166, HUVEC TERT2, LHCN-M2, TIME)
    Figure 9 RNA cell line category: Cell line enhanced (fHDF/TERT166, HUVEC TERT2, LHCN-M2, TIME)
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000137801-THBS1

    Specifications

    • Immunogen
    • Recombinant human THBS1 (AA: 750-850) fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • Human THBS1
    • Species Reactivity
    • Human
    • Clone
    • 5E2E2
    • Molecule Class
    • Matricellular Glycoprotein
    • Applications
    • FC
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS
    • Preservative
    • 0.05% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:500 - 1:2000
      Flow Cytometry: 1:200 - 1:400
      Enzyme-linked Immunosorbent Assay: 1:10000

    Target

    • Alternative Names
    • THBS1; THBS-1; TSP1; Thrombospondin-1p180; Thrombospondin 1; Thrombospondin-1; TSP; TSP-1
    • Long Name
    • THBS1 Gene(Protein Coding) Thrombospondin 1
    • Cellular Localization
    • Endoplasmic reticulum; Extracellular matrix; Sarcoplasmic reticulum; Secreted
    • Post Translation Modifications
    • Disulfide bond; Glycoprotein
    • Function
    • Heparin-binding

    Recommended Products

    Isotype Control

    Secondary Antibody

    Reconstitution Buffer

    Recommended Dilution Buffer

    Related Resources

    • Related Signaling Pathways

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "THBS1"

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    CAT Product Name Application Type
    MOB-1840z Mouse Anti-THBS1 Recombinant Antibody (clone 23D12) WB, ICC, IF, IHC, IP, Neut Mouse IgG1
    FAMAB-0096JF Human Anti-THBS1 Recombinant Antibody (clone TSP-A10) WB, IHC, ICC Human IgG
    ZG-508C Mouse Anti-THBS1 Recombinant Antibody (clone 1.77) ELISA Mouse IgG1, κ
    VS3-WK35 Mouse Anti-THBS1 Recombinant Antibody (clone E2-E2) WB, ELISA Mouse IgG1
    VS3-FY1450 Recombinant Rabbit Anti-THBS1 Antibody (clone R06-5F3) WB, IHC-P, IP Rabbit IgG

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