Mouse Anti-TIGIT Recombinant Antibody (clone 4C5B9)
CAT#: VS7-0425-WR987
This product is a mouse IgG1 antibody that binds to human TIGIT. The antibody 4C5B9 recognizes specific amino acid domain of human TIGIT (AA: extra 22-141). It can be used for FC assay.
Gene Expression
RNA Expression
Figure 1 RNA cell line category: Cell line enhanced (HDLM-2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000181847-TIGIT
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Specifications
- Immunogen
- Recombinant human TIGIT (AA: extra 22-141) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human TIGIT
- Species Reactivity
- Human
- Clone
- 4C5B9
- Molecule Class
- Co-Inhibitory Immune Checkpoint Receptor
- Applications
- FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- TIGIT; T cell immunoreceptor with Ig and ITIM domains; WUCAM; Vstm3
- Gene ID
- 201633
- UniProt ID
- Q495A1
- Long Name
- T cell immunoreceptor with Ig and ITIM domains
- Cellular Localization
- Cell membrane; Membrane
- Post Translation Modifications
- Disulfide bond; Glycoprotein; Phosphoprotein
- Protein Refseq
- NP_776160.2
- Function
- Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells (PubMed:19011627).; Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades (PubMed:23154388).; In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway (PubMed:24817116).; Acts also as a receptor for NECTIN4 to inhibit NK cell cytotoxicity (PubMed:32503945).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
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- Product Name
Secondary Antibody
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Reconstitution Buffer
- CAT
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Recommended Dilution Buffer
- CAT
- Product Name
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