Rabbit Anti-TPBG Recombinant Antibody (clone R05-3A0)
CAT#: VS3-FY1961
The R05-3A0 recombinant monoclonal antibody is specifically created to bind human TPBG. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IP. The binding specificity of the 5T4 antibody was determined using western blot analysis of 5T4 in mouse brain, rat brain lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-TPBG Antibody (VS3-FY1961) in WB.
The 5T4 expression in mouse brain, rat brain lysates were detected using the anti-5T4 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10554/if_selected.jpg
Normal Tissue
Figure 2 Seminal vesicle
High expression in seminal vesicle
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10554/26240_A_7_6_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10554/26240_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10554/26240_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10554/26240_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10554/26240_A_8_5.jpg
Normal Tissue
Figure 7 Placenta
Decidual cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10554/26240_A_2_7.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced.
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000146242-TPBG
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Specifications
- Immunogen
- Synthetic peptide of human 5T4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat TPBG
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-3A0
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 46 kDa, while the observed molecular weight stands at 78 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Trophoblast Glycoprotein; 5T4 Oncofetal Trophoblast Glycoprotein; Wnt-Activated Inhibitory Factor 1; 5T4 Oncotrophoblast Glycoprotein; 5T4 Oncofetal Antigen; WAIF1; M6P1; 5T4; 5T4AG
- Gene ID
- 7162
- UniProt ID
- Q13641
- Cellular Localization
- Cell membrane, Membrane
- Post Translation Modifications
- Highly glycosylated.
- Protein Refseq
- NP_001159864.1; NP_006661.1
- Function
- May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1674z | Mouse Anti-TPBG Recombinant Antibody (clone 28C7) | ELISA, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-118-F(E) | Anti-Human 5T4 Recombinant Antibody Fab Fragment, 5T4 (TAB-118-F(E)) | FuncS, IF, Neut, ELISA, FC, IP, ICC | Fab - G1 - kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1796MZ | Recombinant Mouse Anti-Human TPBG Antibody (clone 3D6) | ELISA, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3649 | Rabbit Anti-TPBG Recombinant Antibody (clone DS3649AB) | WB, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0088CQ | Human Anti-TPBG Recombinant Antibody (HPAB-0088CQ) | ELISA, FC, FuncS | Human IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0089CQ | Human Anti-TPBG Recombinant Antibody (HPAB-0089CQ) | ELISA, FC, FuncS | Human IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0090CQ | Human Anti-TPBG Recombinant Antibody (HPAB-0090CQ) | ELISA, FC, FuncS | Human IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0088CQ-S(P) | Human Anti-TPBG Recombinant Antibody; scFv Fragment (HPAB-0088CQ-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0089CQ-S(P) | Human Anti-TPBG Recombinant Antibody; scFv Fragment (HPAB-0089CQ-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0090CQ-S(P) | Human Anti-TPBG Recombinant Antibody; scFv Fragment (HPAB-0090CQ-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0091CQ-S(P) | Mouse Anti-TPBG Recombinant Antibody; scFv Fragment (HPAB-0091CQ-S(P)) | ELISA, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0092CQ-S(P) | Mouse Anti-TPBG Recombinant Antibody; scFv Fragment (HPAB-0092CQ-S(P)) | ELISA, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0088CQ-F(E) | Human Anti-TPBG Recombinant Antibody; Fab Fragment (HPAB-0088CQ-F(E)) | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0089CQ-F(E) | Human Anti-TPBG Recombinant Antibody; Fab Fragment (HPAB-0089CQ-F(E)) | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0090CQ-F(E) | Human Anti-TPBG Recombinant Antibody; Fab Fragment (HPAB-0090CQ-F(E)) | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0091CQ-F(E) | Mouse Anti-TPBG Recombinant Antibody; Fab Fragment (HPAB-0091CQ-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0092CQ-F(E) | Mouse Anti-TPBG Recombinant Antibody; Fab Fragment (HPAB-0092CQ-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1281 | PE-H-2Db/Human TPBG (ASFRNLTHL) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1282 | APC-H-2Db/Human TPBG (ASFRNLTHL) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1283 | FITC-H-2Db/Human TPBG (ASFRNLTHL) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1284 | H-2Db/Human TPBG (ASFRNLTHL) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY114 | Human Anti-TPBG (clone ASN004) scFv-Fc Chimera | ELISA, FC, FuncS | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY60 | Human Anti-TPBG (clone H8) scFv-Fc Chimera | FC, ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC638 | Recombinant Anti-TPBG Vesicular Antibody, EV Displayed (VS-0425-YC638) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7454 | Anti-TPBG Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7455 | Anti-Mouse TPBG Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC350 | SmartAb™ Recombinant Anti-TPBG pH-dependent Antibody (VS-0825-YC350) | ELISA, FC | Human IgG1 kappa |
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