Rabbit Anti-TPBG Recombinant Antibody (clone R05-3A0) (CAT#: VS3-FY1961)
The R05-3A0 recombinant monoclonal antibody is specifically created to bind human TPBG. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IP. The binding specificity of the 5T4 antibody was determined using western blot analysis of 5T4 in mouse brain, rat brain lysates.
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WB
Figure 1 Anti-TPBG Antibody (VS3-FY1961) in WB.
The 5T4 expression in mouse brain, rat brain lysates were detected using the anti-5T4 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human 5T4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat TPBG
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-3A0
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 46 kDa, while the observed molecular weight stands at 78 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Trophoblast Glycoprotein; 5T4 Oncofetal Trophoblast Glycoprotein; Wnt-Activated Inhibitory Factor 1; 5T4 Oncotrophoblast Glycoprotein; 5T4 Oncofetal Antigen; WAIF1; M6P1; 5T4; 5T4AG
- Gene ID
- 7162
- UniProt ID
- Q13641
- Cellular Localization
- Cell membrane, Membrane
- Post Translation Modifications
- Highly glycosylated.
- Protein Refseq
- NP_001159864.1; NP_006661.1
- Function
- May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-118-F(E) | Anti-Human 5T4 Recombinant Antibody Fab Fragment, 5T4 (TAB-118-F(E)) | FuncS, IF, Neut, ELISA, FC, IP, ICC | Fab - G1 - kappa |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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