Rabbit Anti-TPPP Recombinant Antibody (clone R06-8I4)
CAT#: VS3-FY1920
This R06-8I4 recombinant monoclonal antibody is specifically created to bind human TPPP. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. The specificity of the TPPP antibody was confirmed by western blot analysis of TPPP in mouse brain lysates and immunocytochemistry analysis of TPPP (green) in LNcap using TPPP antibody.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to cytosol & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36576/1719_G6_18_cr5804b9cccd554_selected.jpg
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WB
Figure 1 Anti-TPPP Antibody (VS3-FY1920) in WB.
The TPPP expression in mouse brain lysates were detected using the anti-TPPP by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human tubulin polymerization promoting protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat TPPP
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-8I4
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 24 kDa, while the observed molecular weight stands at 24 kDa.
- Related Disease
- Lymphoid Interstitial Pneumonia and Dementia, Lewy Body
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Tubulin Polymerization Promoting Protein; Brain Specific Protein P25 Alpha; 25 KDa Brain-Specific Protein; P25-Alpha; TPPP/P25; TPPP1
- Gene ID
- 11076
- UniProt ID
- O94811
- Sequence Similarities
- Belongs to the TPPP family.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Golgi apparatus, Nucleus
- Post Translation Modifications
- Phosphorylated by LIMK1 on serine residues; phosphorylation may alter the tubulin polymerization activity (PubMed:17693641, 18028908).
Phosphorylation by LIMK2, but not LIMK1, regulates astral microtubule organization at early stage of mitosis (PubMed:22328514).
Phosphorylation by ROCK1 at Ser-32, Ser-107 and Ser-159 inhibits interaction with HDAC6, resulting in decreased acetylation of tubulin, increased cell motility and entry into S-phase (PubMed:23093407, 23355470).
Phosphorylation by CDK1 inhibits the microtubule polymerizing activity (PubMed:23355470).
Degraded by the proteasome; zinc-binding inhibits degradation by the proteasome.
- Protein Refseq
- XP_016864482.1; XP_024310114.1; XP_047272630.1; XP_054207487.1; XP_054207488.1
- Function
- Acts as a microtubule nucleation factor in oligodendrocytes: specifically localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, and promotes microtubule nucleation, an important step for elongation of the myelin sheath.
Required for both uniform polarized growth of distal microtubules as well as directing the branching of proximal processes.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TPPP"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3660 | Rabbit Anti-TPPP Recombinant Antibody (clone DS3660AB) | WB, IP, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK375 | Rabbit Anti-TPPP Recombinant Antibody (clone JE47-35) | WB, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1182 | CytoStream™ Rabbit Anti-TPPP Recombinant Antibody (VS13-YC1182) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2339 | Anti-TPPP Immunohistochemistry Kit | IHC | |
| VS-0525-XY7472 | Anti-Mouse TPPP Immunohistochemistry Kit | IHC |
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