Rabbit Anti-TPT1 Recombinant Antibody (clone 10A9) (CAT#: ZG-0607U)
Rabbit Anti-TPT1 Antibody (clone 10A9) displayed high specificity against the TPT1 protein of Human and Mouse.
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Custom Production
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WB
Figure 1 Rabbit Anti-TPT1 Antibody (ZG-0607U) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse liver tissue
All lanes: TPT1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 20, 16 kDa
Observed band size: 25 kDa
IHC
Figure 2 Rabbit Anti-TPT1 Antibody (ZG-0607U) in IHC
IHC image of ZG-0607U diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 3 Rabbit Anti-TPT1 Antibody (ZG-0607U) in IF
Immunofluorescence staining of SY5Y Cells with ZG-0607U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
IP
Figure 4 Rabbit Anti-TPT1 Antibody (ZG-0607U) in IP
Immunoprecipitating TCTP in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0607U in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0607U(2µg)+ Hela whole cell lysate(500µg)
Lane 3: Hela whole cell lysate (10µg)
Specifications
- Immunogen
- A synthesized peptide derived from human TCTP
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TPT1
- Species Reactivity
- Human, Mouse
- Clone
- 10A9
- Applications
- ELISA, WB, IHC, IF, IP
- Conjugate
- Unconjugated
- Related Disease
- Chronic Urticaria and Drug Allergy.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000
Target
- Alternative Names
- Translationally-controlled tumor protein; TCTP; Fortilin; Histamine-releasing factor; HRF; p23; TPT1
- Gene ID
- 7178
- UniProt ID
- P13693
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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