Mouse Anti-TRIM28 Recombinant Antibody (VS7-0425-WR1049)

Name: Mouse Anti-TRIM28 Recombinant Antibody (VS7-0425-WR1049)
SKU: VS7-0425-WR1049
Rating: 5 (1 reviews)

CAT#: VS7-0425-WR1049

This product is a mouse IgG antibody that binds to human TRIM28. The antibody can be used for WB assay. It cross reacts with mouse, rat, dog, and rabbit.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line BJ

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/1132_B11_2_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line BJ

Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10066/if_selected.jpg

Subcellular Location Figure 3 IF staining of human cell line BJ

Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_9_7_selected.jpg

Normal Tissue Figure 4 Cerebral cortex

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_B_7_5.jpg

Normal Tissue Figure 5 Colon

Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Fibroblasts
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_7_3.jpg

Normal Tissue Figure 6 Liver

Cholangiocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_7_4.jpg

Normal Tissue Figure 7 Kidney

Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_7_5.jpg

Normal Tissue Figure 8 Testis

Leydig cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining: High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity: 75%-25%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_4_6.jpg

Normal Tissue Figure 9 Skin

Fibroblasts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Keratinocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Langerhans
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Melanocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_B_7_1.jpg

Normal Tissue Figure 10 Lymph node

Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_8_8.jpg

RNA Expression Figure 11 RNA cell line category: Cell line enhanced (HEK93)

Cell lines ordered by descending RNA expression order.

RNA Expression Figure 11 RNA cell line category: Cell line enhanced (HEK93)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000130726-TRIM28

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Specifications

Immunogen
Recombinant human TRIM28 (C-terminus) fragment expressed in E. Coli.

Host Species
Mouse

Type
Mouse IgG

Specificity
Human TRIM28

Species Reactivity
Human, Mouse, Rat, Dog, Rabbit

Molecule Class
Trim Protein

Applications
WB

Conjugate
Unconjugated

Product Property

Clonality
Monoclonal

Purity
>95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid

Format
Liquid

Concentration
1 mg/ml

Buffer
0.1M Tris-Glycine (pH 7.4, 150 mM NaCl)

Preservative
0.2% sodium azide

Storage
Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
Western Blot: 1:1000 - 1:2000

Target

Alternative Names
KAP1; TF1B; RNF96; TIF1B; PPP1R157

Gene ID
10155

UniProt ID
Q13263

Long Name
Tripartite Motif Containing 28

Cellular Localization
Nucleus

Post Translation Modifications
Acetylation; ADP-ribosylation; Citrullination; Isopeptide bond; Phosphoprotein; Ubl conjugation

Protein Refseq
NP_005753.1

Function
Chromatin regulator; Repressor; Transferase

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS7-0425-WR1049.pdf

MSDS

MSDS - VS7-0425-WR1049.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Isotype Control

CAT
Product Name

Secondary Antibody

CAT
Product Name

See other products for "TRIM28"

Select a product category from the dropdown menu below to view related products.

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NABL-054	Recombinant Anti-human TRIM28 VHH Single Domain Antibody	WB, IHC, FC, FuncS	Llama VHH

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CAT	Product Name	Application	Type
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CAT	Product Name	Application
VS-0525-XY7525	Anti-TRIM28 Immunohistochemistry Kit	IHC
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