Mouse Anti-TSG101 Recombinant Antibody (clone 6F4D11) (CAT#: ZG-0259U)

Figure 1 Mouse Anti-TSG101 Antibody (ZG-0259U) in WB

Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate. All lanes: TSG101 antibody at 1: 500
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 44, 32 KDa
Observed band size: 44 KDa
Exposure time: 5min

Figure 2 Mouse Anti-TSG101 Antibody (ZG-0259U) in IF

Immunofluorescence staining of U251 cells with ZG-0259U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Mouse IgG (H+L).

Figure 3 Mouse Anti-TSG101 Antibody (ZG-0259U) in FC

Overlay Peak curve showing U251 cells stained with ZG-0259U (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.