Mouse Anti-TSPAN1 Recombinant Antibody (clone 4/12) (CAT#: VS11-QX142)
This product is a recombinant mouse antibody that recognizes the largest extracellular domain of human TSPAN1. This antibody can be used for the following applications: IF, IHC, ELISA.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11909/201_D5_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11909/201_D5_1_selected.jpg
Figure 2 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows membrane positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11909/27846_A_5_3_selected.jpg
(Immunohistochemical staining of human rectum shows membrane positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11909/27846_A_5_3_selected.jpg
Figure 3 IF staining of human cell line RT4
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11909/1386_D4_2_red_green.jpg
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11909/1386_D4_2_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11909/161_A4_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11909/161_A4_1_red_green.jpg
Figure 5 Cerebral cortex
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11909/27846_B_7_5.jpg
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11909/27846_B_7_5.jpg
Figure 6 Colon
(Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11909/27846_A_7_3.jpg
(Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11909/27846_A_7_3.jpg
Figure 7 Kidney
(Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11909/27846_A_9_5.jpg
(Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11909/27846_A_9_5.jpg
Figure 8 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11909/27846_A_5_6.jpg
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11909/27846_A_5_6.jpg
Figure 9 RNA cell line category: Cell line enhanced (A-431, CAPAN-2, HaCaT, hTCEpi, OE19, RPTEC TERT1, RT4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000117472-TSPAN1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000117472-TSPAN1
Specifications
- Immunogen
- The major extracellular domain of tetraspanin 1
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human TSPAN1
- Species Reactivity
- Human
- Clone
- 4/12
- Applications
- IF, IHC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- 1 mg/mL (Please refer to the vial label for the specific concentration.)
- Buffer
- 0.2 μm filtered solution in PBS
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C.
Target
- Alternative Names
- NET1; TM4C; TM4SF
- Gene ID
- 10103
- UniProt ID
- O60635
- Long Name
- Tetraspanin-1
- Sequence Similarities
- Belongs to the tetraspanin (TM4SF) family.
- Cellular Localization
- Nucleoplasm, Vesicles
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Single Domain Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1377-VHH | Recombinant Anti-human TSPAN1 VHH Single Domain Antibody | WB, IP, ICC, FC, FA | Llama VHH |
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