Mouse Anti-TUBA1A Recombinant Antibody (clone 7E5C12) (CAT#: ZG-0286U)

Figure 1 Mouse Anti-TUBA1A Antibody (ZG-0286U) in WB

Positive WB detected in: U87 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate, SH-SY5Y whole cell lysate. All lanes: TUBA1A antibody at 1: 5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

Figure 2 Mouse Anti-TUBA1A Antibody (ZG-0286U) in IHC

IHC image of ZG-0286U diluted at 1:150 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 3 Mouse Anti-TUBA1A Antibody (ZG-0286U) in IF

Immunofluorescence staining of Hela cells with ZG-0286U at 1:75, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).

Figure 4 Mouse Anti-TUBA1A Antibody (ZG-0286U) in IP

Immunoprecipitating TUBA1A in Hela whole cell lysate Lane 1: Mouse control IgG (1µg) in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0286U (5µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)

Figure 5 Mouse Anti-TUBA1A Antibody (ZG-0286U) in FC

Overlay histogram showing Hela cells stained with ZG-0286U (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.