Rabbit Anti-TUBB Polyclonal Antibody
CAT#: MRO-0204YJ
This product is a polyclonal rabbit antibody that recognizes TUBB.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5417/968_C12_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules & cytokinetic bridge.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43640/1773_E7_6_selected.jpg
Normal Tissue
Figure 3 IHC staining of human breast
Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5417/ihc_selected.jpg
Normal Tissue
Figure 4 Ovary.
High expression in ovary
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12406/31977_A_5_7_val_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5417/19754_A_8_8.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12406/31977_A_9_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12406/31977_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12406/31977_A_5_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196230-TUBB
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Specifications
- Immunogen
- A synthesized peptide derived from human Tubulin beta.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TUBB
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC, IF, ICC, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, pH 7.4, 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:1000-1:5000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
ELISA: 1:20000-1:40000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "TUBB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0911MZ | Recombinant Mouse Anti-Human TUBB Antibody (clone 48C) | ELISA, IHC-P, WB | Mouse antibody |
| ZG-0294U | Mouse Anti-TUBB Recombinant Antibody (clone 16E11D4) | ELISA, WB, IHC, IF, FC, IP | Mouse IgG2b |
| VS3-QX1193 | Mouse Anti-TUBB Recombinant Antibody (clone 10B7-1G4-7E1) | WB, IFIHC | Mouse IgG1 |
| VS3-QX1194 | Mouse Anti-TUBB Recombinant Antibody (clone 3D8-4E2-3F10) | WB, IHC, IP | Mouse IgG1 |
| VS3-QX1195 | Mouse Anti-TUBB Recombinant Antibody (clone 6H2-4E6-8G2) | WB, IHC, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3721 | Rabbit Anti-TUBB Recombinant Antibody (clone DS3721AB) | WB, IHC, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0816LC | Mouse Anti-TUBB Recombinant Antibody (clone TU-06) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1191 | CytoStream™ Rabbit Anti-TUBB Recombinant Antibody (VS13-YC1191) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7612 | Anti-TUBB Immunohistochemistry Kit | IHC | |
| VS-0525-XY7613 | Anti-Mouse TUBB Immunohistochemistry Kit | IHC | |
| VS-0525-XY7614 | Anti-Rat TUBB Immunohistochemistry Kit | IHC | |
| VS-0525-XY7615 | Anti-Chicken TUBB Immunohistochemistry Kit | IHC |
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