Mouse Anti-TUBB Recombinant Antibody (clone 16E11D4) (CAT#: ZG-0294U)

Figure 1 Mouse Anti-TUBB Antibody (ZG-0294U) in WB

Positive WB detected in: 293 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate All lanes TUBB antibody at 1: 5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 55 KDa
Observed band size: 55 KDa
Exposure time: 5s

Figure 2 Mouse Anti-TUBB Antibody (ZG-0294U) in IHC

IHC image of ZG-0294U diluted at 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Figure 3 Mouse Anti-TUBB Antibody (ZG-0294U) in IF

Immunofluorescence staining of Hela cells with ZG-0294U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Mouse IgG(H+L).

Figure 4 Mouse Anti-TUBB Antibody (ZG-0294U) in FC

Overlay Peak curve showing NIH/3T3 cells stained with ZG-0294U (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Figure 5 Mouse Anti-TUBB Antibody (ZG-0294U) in IP

Immunoprecipitating TUBB in Hela whole cell lysate. Lane 1: Mouse control IgG in Hela whole cell lysate.
Lane 2: ZG-0294U (2µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (5µg)
For western blotting, the blot was detected with ZG-0294U at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:5000