Mouse Anti-UBB Recombinant Antibody (clone 5F1)
CAT#: VS3-WK1579
This antibody is a mouse recombinant antibody against human UBB, which is involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. The clone 5F1 can be used in WB analysis at a dilution of 1/1000-2000, IF analysis at a dilution of 1/200, and IHC analysis at a dilution of 1/100-200.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human oral mucosa
Immunohistochemical staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41344/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/116924_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49132/117232_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170315-UBB/subcellular
❮
❯
Specifications
- Immunogen
- Synthetic peptide of Ubiquitin
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human UBB
- Species Reactivity
- Human, Rat, Mouse
- Clone
- 5F1
- Applications
- WB, IF, ICC, IHC-P
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.5% BSA, 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/1000-1/2000
IHC: 1/100-1/200
IF: 1/200
Target
- Alternative Names
- Ubiquitin B; Polyubiquitin B; Epididymis Secretory Protein Li 50; Polyubiquitin-B; HEL-S-50
- Gene ID
- 7314
- UniProt ID
- P0CG47
- Long Name
- Ubiquitin B
- Sequence Similarities
- Belongs to the ubiquitin family.
- Cellular Localization
- Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus
- Post Translation Modifications
- Ubiquitin:
Phosphorylated at Ser-65 by PINK1 during mitophagy (PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25527291, PubMed:26161729).
Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy (PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25527291, PubMed:26161729).
Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30 (PubMed:25527291).
Mono-ADP-ribosylated at the C-terminus by PARP9, a component of the PPAR9-DTX3L complex. ADP-ribosylation requires processing by E1 and E2 enzymes and prevents ubiquitin conjugation to substrates such as histones.
(Microbial infection) Mono-ADP-ribosylated at Thr-66 by the C.violaceum CteC virulence factor. ADP-ribosylation causes the shutdown of polyubiquitin synthesis and disrupts the recognition and reversal of polyubiquitin.
- Protein Refseq
- NP_001268645.1; NP_001268646.1; NP_001268647.1N
- Function
- Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains).
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-WK1579. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
Isotype Control
- CAT
- Product Name
See other products for "UBB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1021 | Recombinant Anti-human UBB Antibody | ELISA, WB, Dot, FuncS | IgG |
| MHH-1021 | Recombinant Human Anti-human UBB Antibody | ELISA, IF, FuncS | IgG |
| MRO-1551-CN | Rabbit Anti-UBB Recombinant Antibody (clone CBACN-570) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ552 | Rabbit Anti-UBB Recombinant Antibody (VS3-CJ552) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS3-FY1507 | Recombinant Rabbit Anti-UBB Antibody (clone R06-2H7) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1021-F(E) | Recombinant Anti-human UBB Antibody Fab Fragment | IP, FuncS | Fab |
| MHH-1021-F(E) | Recombinant Human Anti-human UBB Antibody Fab Fragment | WB, IF, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1021-S(P) | Recombinant Anti-human UBB Antibody scFv Fragment | IF, Neut, FuncS | scFv |
| MHH-1021-S(P) | Recombinant Human Anti-human UBB Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3746 | Rabbit Anti-UBB Recombinant Antibody (clone DS3746AB) | WB, IHC | Rabbit IgG |
| MOR-4756 | Rabbit Anti-UBB Recombinant Antibody (clone TH270DS) | WB, IF, ICC, ChIP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1200 | CytoStream™ Rabbit Anti-UBB Recombinant Antibody (VS13-YC1200) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2412 | Anti-UBB Immunohistochemistry Kit | IHC | |
| VS-0525-XY7687 | Anti-Human UBB Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, WB
-5.jpg)
Application: ELISA, FC, IF, WB
Application: ELISA, IHC, FC, IP, IF, FuncS
-3.jpg)
Application: Neut
Application: Inhib, FC, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
