Mouse Anti-UCHL1 Recombinant Antibody (VS3-CJ548)
CAT#: VS3-CJ548
This product is a mouse antibody that recognizes human, mouse, and rat UCHL1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5993/1902_A11_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/665_E11_1_selected.jpg
Normal Tissue
Figure 3 Kidney
Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_8_5.jpg
Normal Tissue
Figure 4 Testis
Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_6_6.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25% Peripheral nerve/ganglion Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_9_3.jpg
Normal Tissue
Figure 7 Pancreas
Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_1_3.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (EFO-21, SCLC-21H, U-251 MG, U-266/84, U-87 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000154277-UCHL1
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Specifications
- Immunogen
- Recombinant protein of UCHL1
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human, Mouse, Rat UCHL1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IF, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.3.
- Preservative
- 0.09% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:1000), Immunofluorescence (1:25), Immunohistochemistry-Parraffin (1:25).
Target
- Alternative Names
- NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
- Gene ID
- 7345
- UniProt ID
- P09936
- Sequence Similarities
- Belongs to the peptidase C12 family.
- Cellular Localization
- Cytoplasm, Endoplasmic reticulum, Membrane
- Post Translation Modifications
- O-glycosylated.
- Protein Refseq
- NP_004172.2
- Function
- Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "UCHL1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1897MZ | Recombinant Mouse Anti-Human UCHL1 Antibody (clone 20B2) | IHC-Fr, IHC-P, WB | Mouse antibody |
| VS3-QX1215 | Mouse Anti-UCHL1 Recombinant Antibody (clone 7B8-G5-E7) | WB, ICC | Mouse IgG2b |
| VS3-QX1216 | Mouse Anti-UCHL1 Recombinant Antibody (clone 696CT2.1.4) | WB, IHC | Mouse IgG1 |
| VS3-CJ546 | Mouse Anti-UCHL1 Recombinant Antibody (clone ABT-PGP9.5) | WB, IHC-P | Mouse IgG1, κ |
| VS-0723-WK91 | Rabbit Anti-UCHL1 Recombinant Antibody (VS-0723-WK91) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0615MZ | Chicken Anti-UCHL1 Polyclonal IgY | WB | Chicken antibody |
| BRD-0800MZ | Chicken Anti-PGP9.5 Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3771 | Rabbit Anti-UCHL1 Recombinant Antibody (clone DS3771AB) | WB, IP, IHC-P, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY270 | AbPlus™ Anti-UCHL1 Magnetic Beads (JF93-08) | IP, Protein Purification | |
| VS-0724-YC1162 | AbPlus™ Anti-Uchl1 Magnetic Beads (VS-0724-YC1162) | IP, Protein Purification | |
| VS-0724-YC1164 | AbPlus™ Anti-UCHL1 Magnetic Beads (VS-0724-YC1164) | IP, Protein Purification | |
| VS-0724-YC1237 | AbPlus™ Anti-Uchl1 Magnetic Beads (VS-0724-YC1237) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2427 | Anti-UCHL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7722 | Anti-Mouse UCHL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7723 | Anti-Rat UCHL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7721 | Anti-Human UCHL1 Immunohistochemistry Kit | IHC |
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